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PMID: 1712357 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Role and expression of the Bacillus subtilis rodC operon.

Journal of bacteriology ·Vol. 173 ·No. 14 ·1991-07-00 ·Pages 4341-6

Wagner PM, Stewart GC

Abstract

The role of the rodC operon in Bacillus subtilis was investigated. The operon encodes two genes (rodD and rodC) necessary for the synthesis of the cell wall teichoic acid. Transcription of this operon is responsive to levels of phosphate and to concentrations of magnesium ions in the growth medium. This regulation of mRNA production corresponds to conditions that dictate the type of polymer that will be synthesized for the cell wall, i.e., teichoic or teichuronic acid. While the introduction of multiple copies of rodC was tolerated by the cells, multiple copies of rodD appeared to be lethal. The lethality of the rodD fragment was not exhibited if multiple copies of rodC were also present.

Related Genes
rod C rod D
MeSH Terms
Bacillus subtilis/genetics,growth & development,ultrastructure Cell Wall/physiology Escherichia coli/genetics Genes, Bacterial Microscopy, Electron, Scanning Operon Protein Biosynthesis RNA, Bacterial/genetics,isolation & purification Restriction Mapping Teichoic Acids/biosynthesis Transformation, Bacterial
Chemicals
RNA, Bacterial Teichoic Acids
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wagner P M
Department of Microbiology, University of Kansas, Lawrence 66045.
Stewart G C
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27 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.14.4341-4346.1991
Published
1991-07-00
Pages
4341-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208094
Subset
IM
Grants
NIGMS NIH HHS · GM37990 · United States
External Links
PubMed source DOI: 10.1128/jb.173.14.4341-4346.1991 PMC Full Text View journal details
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