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PMID: 8386283 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The 23-kilodalton E1 phosphoprotein of bovine papillomavirus type 1 is nonessential for stable plasmid replication in murine C127 cells.

Journal of virology ·Vol. 67 ·No. 5 ·1993-05-00 ·Pages 2932-7

Hubert WG, Lambert PF

Abstract

The 23-kDa protein encoded by the 5' segment of the E1 open reading frame of bovine papillomavirus type 1 (BPV1) was previously ascribed a negative regulatory function for the replication of viral plasmid DNA. However, results from recent functional and biochemical studies do not readily support this genetic assignment. Therefore, we have reassessed the role of this protein in papillomavirus DNA replication by using a mutant of BPV1 which is unable to express this E1 protein. This mutant viral DNA was found to replicate extrachromosomally with stability and copy number per cell similar to those of wild-type plasmid DNA. Thus, the absence of expression of the 23-kDa E1 protein did not lead to deregulated viral plasmid replication. We conclude that the 23-kDa E1 protein is nonessential for stable plasmid replication.

MeSH Terms
Amino Acid Sequence Antibodies, Viral Base Sequence Bovine papillomavirus 1/genetics,growth & development,pathogenicity DNA Replication/genetics DNA, Viral/genetics DNA-Binding Proteins/genetics,immunology Drug Resistance, Microbial Gentamicins/pharmacology Molecular Sequence Data Plasmids Precipitin Tests RNA, Viral/genetics Selection, Genetic Serial Passage Transfection Viral Proteins/genetics,immunology Virus Replication
Chemicals
Antibodies, Viral DNA, Viral DNA-Binding Proteins E1 protein, Bovine papillomavirus Gentamicins RNA, Viral Viral Proteins antibiotic G 418
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hubert W G
McArdle Laboratory for Cancer Research, University of Wisconsin, Madison 53706.
Lambert P F
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.5.2932-2937.1993
Published
1993-05-00
Pages
2932-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237622
Subset
IM
Grants
NCI NIH HHS · CA07175 · United States
NCI NIH HHS · CA09135 · United States
NCI NIH HHS · CA55048 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.5.2932-2937.1993 PMC Full Text View journal details
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