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PMID: 8385772 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of the cytosolic pH set point for activation of the Na+/H+ antiport in human platelets: the roles of the Na+/Ca2+ exchange, the Na(+)-K(+)-2Cl- cotransport and cellular volume.

Pflugers Archiv : European journal of physiology ·Vol. 422 ·No. 6 ·1993-03-00 ·Pages 585-90

Kimura M, Aviv A

Abstract

To explore further the mechanisms that regulate the Na+/H+ antiport in human platelets, we examined the effect of Na+ pump inhibition by ouabain and K+ removal from the extracellular medium on parameters of this transport system. Treatment with ouabain resulted in increased cytosolic free Ca2+ and Na+, coupled with an alkaline shift in the cytosolic pH set point for the Na+/H+ antiport. Inhibition of the Na+ pump by the removal of K+ from the medium increased the cytosolic Na+ but not the cytosolic Ca2+; yet this treatment also produced a substantial alkaline shift in the cytosolic pH set point for the Na+/H+ antiport. This effect appeared to relate to a decline in cellular volume and it was attenuated by the Na(+)-K(+)-2 Cl- cotransport inhibitor, bumetanide. These findings indicate: (a) a link between the Na+ pump and the Na+/H+ antiport, mediated by the Na+/Ca2+ exchange and the cytosolic free Ca2+, and (b) a link between the Na+/H+ antiport and the Na(+)-K(+)-2Cl- cotransport through cellular volume.

MeSH Terms
Blood Platelets/drug effects,metabolism Bumetanide/pharmacology Calcium/blood Carrier Proteins/blood Cytosol/metabolism Humans Hydrogen-Ion Concentration Ouabain/pharmacology Potassium Chloride/pharmacology Sodium/blood Sodium-Calcium Exchanger Sodium-Hydrogen Exchangers Sodium-Potassium-Chloride Symporters Sodium-Potassium-Exchanging ATPase/drug effects
Chemicals
Carrier Proteins Sodium-Calcium Exchanger Sodium-Hydrogen Exchangers Sodium-Potassium-Chloride Symporters Bumetanide Ouabain Potassium Chloride Sodium Sodium-Potassium-Exchanging ATPase Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kimura M
Hypertension Research Center, University of Medicine and Dentistry of NJ-NJ Medical School, Newark 07103-2714.
Aviv A
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00374006
Published
1993-03-00
Pages
585-90
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
Grants
NHLBI NIH HHS · HL34807 · United States
NHLBI NIH HHS · HL42856 · United States
External Links
PubMed source DOI: 10.1007/BF00374006 View journal details
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