Cytoplasmic acidification and activation of Na+/H+ exchange during regulatory volume decrease in Ehrlich ascites tumor cells.

The Journal of membrane biology ·Vol. 114 ·No. 2 ·1990-03-00 ·Pages 153-7

Livne A, Hoffmann EK

Abstract

Ehrlich ascites tumor cells undergoing regulatory volume decrease (RVD) exhibit cytoplasmic acidification as measured by an intracellular fluorescent pH indicator. The acidification results in an activation of the Na+/H+ exchanger. The intracellular pH 'set point' for the activation is estimated to be around 7.0. The activation of the Na+H+ exchanger leads to an incomplete RVD. In support of this conclusion, amiloride and Na+-free medium, known to limit the Na+/H+ exchange, indeed enhance the RVD response. Intracellular acidification and activation of Na+/H+ exchange may be a general response of cells undergoing RVD.

MeSH Terms

Amiloride/pharmacology Animals Carcinoma, Ehrlich Tumor/metabolism,pathology Carrier Proteins/metabolism Cytoplasm/metabolism Hydrogen-Ion Concentration Ion Exchange Mice Osmolar Concentration Protons Sodium/metabolism Sodium-Hydrogen Exchangers Tumor Cells, Cultured

Chemicals

Carrier Proteins Protons Sodium-Hydrogen Exchangers Amiloride Sodium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Livne A

Institute of Biological Chemistry, University of Copenhagen, Denmark.

Hoffmann E K

References (14)

14 references, click to expand

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Article Info

Journal

The Journal of membrane biology

Abbr.

J Membr Biol

ISSN

0022-2631

DOI

10.1007/BF01869096

Published

1990-03-00

Pages

153-7

Language

English

Region

United States

NLM ID

0211301

Subset

External Links

PubMed source DOI: 10.1007/BF01869096 View journal details

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Cytoplasmic acidification and activation of Na+/H+ exchange during regulatory volume decrease in Ehrlich ascites tumor cells.

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