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PMID: 8382609 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterodimer formation of cJun and ATF-2 is responsible for induction of c-jun by the 243 amino acid adenovirus E1A protein.

The EMBO journal ·Vol. 12 ·No. 2 ·1993-02-00 ·Pages 479-87

van Dam H, Duyndam M, Rottier R, Bosch A, de Vries-Smits L, Herrlich P, Zantema A, Angel P, van der Eb AJ

Abstract

The adenovirus E1A proteins differentially regulate AP-1-responsive genes. Collagenase and stromelysin are repressed by E1A, whereas the expression of c-jun is elevated. Inhibition of collagenase has been found to be exerted through the consensus AP-1 binding site TGAGTCA. Here we show that the distal AP-1 binding site in the c-jun promoter, the jun2TRE (TTACCTCA), is the decisive element of this promoter in mediating the positive response to the 243 amino acid E1A product. In vitro binding studies revealed that, in contrast to the consensus AP-1 site which is preferentially targeted by dimers composed of the Jun and Fos families, the jun2TRE binds heterodimers composed of cJun and ATF-2(-like) proteins. Since stimulation of c-jun transcription is a function of the transforming domain of E1A encoded by conserved region 1, cJun--ATF-2 may be one of the effector factors involved in transformation. The data further suggest that E1A can distinguish between cJun--cJun and cJun--ATF-2 in imposing opposite states of activity.

Related Genes
c-jun
MeSH Terms
3T3 Cells Activating Transcription Factors Adenovirus E1A Proteins/metabolism Animals Base Sequence Binding Sites Blood Proteins/metabolism Cyclic AMP/metabolism Genes, jun HeLa Cells Humans Mice Molecular Sequence Data Oligonucleotides Proto-Oncogene Proteins c-jun/genetics,metabolism Transcription Factors/metabolism Transcription, Genetic
Chemicals
Activating Transcription Factors Adenovirus E1A Proteins Blood Proteins Oligonucleotides Proto-Oncogene Proteins c-jun Transcription Factors Cyclic AMP
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
van Dam H
Laboratory for Molecular Carcinogenesis, Sylvius Laboratories, University of Leiden, The Netherlands.
Duyndam M
Rottier R
Bosch A
de Vries-Smits L
Herrlich P
Zantema A
Angel P
van der Eb A J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-02-00
Pages
479-87
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413231
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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