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PMID: 8349564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of twenty-six new heat shock genes of Escherichia coli.

Journal of bacteriology ·Vol. 175 ·No. 16 ·1993-08-00 ·Pages 5242-52

Chuang SE, Blattner FR

Abstract

Most organisms respond to heat by substantial alteration of the pattern of gene expression. This has been particularly well studied with Escherichia coli although the response has by no means been completely characterized. Here we report the characterization of 26 new heat shock genes of E. coli, termed hsl, discovered by global transcription analysis with an overlapping lambda clone bank. We have measured the molecular weights of the corresponding heat shock proteins and mapped each of them to within a few kilobases on the E. coli genome. In vitro, 16 of them can be activated by the E sigma 32 RNA polymerase, which specifically transcribes heat shock genes. In vivo expression kinetics of seven of eight examined new proteins were found to be similar to those of the four most studied heat shock proteins, DnaK, DnaJ, GroEL (MopA), and GroES (MopB). In the course of this work, we confirmed that the catalytic subunit of the ATP-dependent Clp protease (also known as Ti protease), ClpP, is derived from a larger precursor protein. Possible assignments of some of the hsl genes to known proteins are discussed.

MeSH Terms
Bacterial Proteins/biosynthesis,genetics Bacteriophage lambda/genetics Chromosome Mapping Cloning, Molecular Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial/genetics Heat-Shock Proteins/biosynthesis,genetics,metabolism Recombinant Proteins/biosynthesis Sigma Factor/metabolism Transcription Factors Transcription, Genetic/radiation effects Ultraviolet Rays
Chemicals
Bacterial Proteins Heat-Shock Proteins Recombinant Proteins Sigma Factor Transcription Factors heat-shock sigma factor 32
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chuang S E
Laboratory of Genetics, University of Wisconsin-Madison 53706.
Blattner F R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.16.5242-5252.1993
Published
1993-08-00
Pages
5242-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204992
Subset
IM
Grants
NIGMS NIH HHS · GM35682 · United States
External Links
PubMed source DOI: 10.1128/jb.175.16.5242-5252.1993 PMC Full Text View journal details
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