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PMID: 1849513 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Increased ATP-dependent proteolytic activity in lon-deficient Escherichia coli strains lacking the DnaK protein.

Journal of bacteriology ·Vol. 173 ·No. 8 ·1991-04-00 ·Pages 2691-5

Kroh HE, Simon LD

Abstract

Extracts made from Escherichia coli null dnaK strains contained elevated levels of ATP-dependent proteolytic activity compared with levels in extracts made from dnaK+ strains. This ATP-dependent proteolytic activity was not due to Lon, Clp, or Alp-associated protease. Comparison of the levels of ATP-dependent proteolytic activity present in lon rpoH dnaK mutants and in lon rpoH dnaK+ mutants showed that the level of ATP-dependent proteolytic activity was elevated in the lon rpoH dnaK mutant strain. These findings suggest that DnaK negatively regulates a new ATP-dependent proteolytic activity, independently of sigma 32. Other results indicate that an ATP-dependent proteolytic activity was increased in a lon alp strain after heat shock. It is not yet known whether the same protease is associated with the increased ATP-dependent proteolytic activity in the dnaK mutants and in the heat-shocked lon alph strain.

MeSH Terms
ATP-Dependent Proteases Adenosine Triphosphate/physiology DNA, Circular/physiology DNA, Kinetoplast Escherichia coli/physiology Escherichia coli Proteins Gene Expression Regulation, Bacterial Heat-Shock Proteins/physiology Hydrolysis Protease La Serine Endopeptidases/deficiency
Chemicals
DNA, Circular DNA, Kinetoplast Escherichia coli Proteins Heat-Shock Proteins Adenosine Triphosphate ATP-Dependent Proteases Serine Endopeptidases Lon protein, E coli Protease La
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kroh H E
Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08854.
Simon L D
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.8.2691-2695.1991
Published
1991-04-00
Pages
2691-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207838
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.8.2691-2695.1991 PMC Full Text View journal details
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