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PMID: 8341710 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 14 ·1993-07-15 ·Pages 6859-63

Ogawa E, Maruyama M, Kagoshima H, Inuzuka M, Lu J, Satake M, Shigesada K, Ito Y

Abstract

cDNAs representing the alpha subunit of polyomavirus enhancer binding protein 2 (PEBP2; also called PEA2) were isolated. The products of the cDNAs are highly homologous to that of Drosophila segmentation gene runt (run) for an N-proximal 128-amino acid region showing 66% identity. The run homology region encompasses the domain capable of binding to a specific nucleotide sequence motif and of dimerizing with the companion beta subunit. The human AML1 gene related to t(8;21) acute myeloid leukemia also had a run homology region. Together with the beta subunit, which increases the affinity of the alpha subunit to DNA without binding to DNA by itself, PEBP2 represents a newly discovered family of transcription factor. The major species of PEBP2 alpha mRNA was expressed in T-cell lines but not in B-cell lines tested. Evidence indicated that PEBP2 functions as a transcriptional activator and is involved in regulation of T-cell-specific gene expression.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Core Binding Factor Alpha 2 Subunit Core Binding Factor alpha Subunits DNA-Binding Proteins/genetics Drosophila/genetics Drosophila Proteins Mice Molecular Sequence Data Multigene Family/genetics Neoplasm Proteins/genetics Nuclear Proteins Proto-Oncogene Proteins RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Sequence Analysis Sequence Homology, Amino Acid Transcription Factor AP-2 Transcription Factors/genetics,metabolism
Chemicals
Core Binding Factor Alpha 2 Subunit Core Binding Factor alpha Subunits DNA-Binding Proteins Drosophila Proteins Neoplasm Proteins Nuclear Proteins Proto-Oncogene Proteins RNA, Messenger Runx1 protein, mouse TfAP-2 protein, Drosophila Transcription Factor AP-2 Transcription Factors run protein, Drosophila
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ogawa E
Department of Viral Oncology, Kyoto University, Japan.
Maruyama M
Kagoshima H
Inuzuka M
Lu J
Satake M
Shigesada K
Ito Y
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.14.6859
Published
1993-07-15
Pages
6859-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47032
Subset
IM
Databases
GENBANK
D14636, D14637
External Links
PubMed source DOI: 10.1073/pnas.90.14.6859 PMC Full Text View journal details
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