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PMID: 1325429 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Differential expression of subspecies of polyomavirus and murine leukemia virus enhancer core binding protein, PEBP2, in various hematopoietic cells.

Japanese journal of cancer research : Gann ·Vol. 83 ·No. 7 ·1992-07-00 ·Pages 714-22

Satake M, Inuzuka M, Shigesada K, Oikawa T, Ito Y

Abstract

The core sequence of the enhancer of murine leukemia virus (MuLV) long terminal repeat is highly conserved in a large number of MuLV strains and appears to play an essential role when SL3-3 or Moloney strains induce T cell lymphoma in mice. We found by using the electrophoretic mobility shift assay that a polyomavirus enhancer core-binding protein, PEBP2, bound to this core motif of MuLV. We also noted that PEBP2 in several hematopoietic cell lines derived from B lymphocyte, macrophage and myelocyte lineages migrated significantly faster than the authentic PEBP2 detected in NIH3T3 fibroblasts. Interestingly, PEBP2 detected in the cell lines of T lymphocyte lineage appeared to contain both types, which were indistinguishable in electrophoretic mobility from those of NIH3T3 and of B lymphocyte, macrophage and myelocyte lineages. The treatment of the nuclear extract containing PEBP2 with phosphatase generated PEBP3, which is a subcomponent of PEBP2 and retained the same DNA-binding specificity as PEBP2. The altered mobility of hematopoietic cell-derived or T lymphocyte-derived PEBP2 was found to be due to the alteration of the mobility of PEBP3. Based on the distinct mobility of PEBP2/3 of T lymphocytes from those of other hematopoietic cells, we discuss the implication of PEBP2 in MuLV-induced T cell leukemia and T cell-specific gene expression.

MeSH Terms
3T3 Cells/microbiology,physiology Acid Phosphatase/pharmacology Animals B-Lymphocytes/microbiology,physiology Base Sequence Binding Sites Core Binding Factor Alpha 1 Subunit DNA-Binding Proteins/genetics,metabolism,physiology Electrophoresis Enhancer Elements, Genetic/genetics,physiology Gene Expression/genetics Genetic Variation/genetics Hematopoietic System/cytology,metabolism,physiology Leukemia L1210/genetics,microbiology,pathology Mice Molecular Sequence Data Moloney murine leukemia virus/genetics,metabolism Polyomavirus/genetics,metabolism T-Lymphocytes/microbiology,physiology Transcription Factor AP-2 Transcription Factors/genetics,metabolism,physiology Viral Core Proteins/genetics,metabolism,physiology
Chemicals
Core Binding Factor Alpha 1 Subunit DNA-Binding Proteins Runx2 protein, mouse Transcription Factor AP-2 Transcription Factors Viral Core Proteins enhancer-binding protein AP-3 Acid Phosphatase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Satake M
Department of Viral Oncology, Kyoto University.
Inuzuka M
Shigesada K
Oikawa T
Ito Y
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Article Info
Journal
Japanese journal of cancer research : Gann
Abbr.
Jpn J Cancer Res
ISSN
0910-5050
DOI
10.1111/j.1349-7006.1992.tb01971.x
Published
1992-07-00
Pages
714-22
Language
English
Region
Japan
NLM ID
8509412
PMCID
PMC5918933
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1349-7006.1992.tb01971.x PMC Full Text
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