Home LiteratureArticle Details
PMID: 8332496 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of H3.3 replacement histone mRNAs during the precommitment period of murine erythroleukemia cell differentiation.

Nucleic acids research ·Vol. 21 ·No. 12 ·1993-06-25 ·Pages 2873-9

Krimer DB, Cheng G, Skoultchi AI

Abstract

Differential hybridization to a cDNA library made from the mRNA of differentiating mouse erythroleukemia (MEL) cells has been used to identify sequences that are induced during the early stages of MEL cell differentiation. One of the differentially expressed genes identified encodes the H3.3 histone subtype. We show here that the three polyadenylated mRNAs produced from the H3.3B gene, as well as the single mRNA produced from the related H3.3A gene, are coordinately induced during the first few hours of MEL cell differentiation and subsequently down regulated as cells undergo terminal differentiation. Nuclear run-on transcription experiments indicate that the accumulation and decay of these mRNAs are controlled at the post-transcriptional level. Unlike the polyadenylated mRNAs of two H1 histone genes that exhibit similar kinetics of induction and decay controlled by c-myc, induction of the H3.3 mRNAs is unaffected by deregulated expression of c-myc.

Related Genes
c-myb c-myc
MeSH Terms
Animals Animals, Newborn/metabolism Blotting, Northern Cell Differentiation DNA Probes Gene Expression Genes, myc Genetic Variation Histones/genetics Leukemia, Erythroblastic, Acute/metabolism,pathology Mice Nucleic Acid Hybridization Oncogenes RNA, Messenger/metabolism Transfection Tumor Cells, Cultured
Chemicals
DNA Probes Histones RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krimer D B
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461.
Cheng G
Skoultchi A I
References (27)
27 references, click to expand
  1. Transcriptional and post-transcriptional regulation of globin gene accumulation in murine erythroleukemia cells.
    Mol Cell Biol. 1983 Feb;3(2):229-32 PMID: 6572784
  2. Extreme sequence conservation characterizes the rabbit H3.3A histone cDNA.
    Nucleic Acids Res. 1990 May 25;18(10):3075 PMID: 2349118
  3. Histone complements of human tissues, carcinomas, and carcinoma-derived cell lines.
    Mol Cell Biochem. 1984 Nov;65(1):57-66 PMID: 6542967
  4. Structure of a human histone cDNA: evidence that basally expressed histone genes have intervening sequences and encode polyadenylylated mRNAs.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2834-8 PMID: 2859593
  5. Replacement variant histone genes contain intervening sequences.
    Mol Cell Biol. 1985 Jun;5(6):1307-17 PMID: 2863747
  6. Absolute rates of globin gene transcription and mRNA formation during differentiation of cultured mouse erythroleukemia cells.
    J Biol Chem. 1985 Oct 5;260(22):12167-73 PMID: 3862667
  7. Constitutive c-myc oncogene expression blocks mouse erythroleukaemia cell differentiation but not commitment.
    Nature. 1986 Apr 24-30;320(6064):760-3 PMID: 3458027
  8. Regulated expression of a chimeric histone gene introduced into mouse fibroblasts.
    Mol Cell Biol. 1985 Sep;5(9):2316-24 PMID: 2874481
  9. Changes in the levels of three different classes of histone mRNA during murine erythroleukemia cell differentiation.
    Mol Cell Biol. 1985 Nov;5(11):2879-86 PMID: 3018484
  10. Expression of a transfected human c-myc oncogene inhibits differentiation of a mouse erythroleukaemia cell line.
    Nature. 1986 Aug 21-27;322(6081):748-50 PMID: 3528861
  11. Deregulated expression of c-myc by murine erythroleukaemia cells prevents differentiation.
    Nature. 1986 Aug 28-Sep 3;322(6082):848-50 PMID: 3528863
  12. Transfection of mouse erythroleukemia cells with myc sequences changes the rate of induced commitment to differentiate.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6480-4 PMID: 3529085
  13. Changes in gene expression associated with induced differentiation of erythroleukemia: protooncogenes, globin genes, and cell division.
    Proc Natl Acad Sci U S A. 1986 Sep;83(18):6849-53 PMID: 3462732
  14. Changes in gene expression during hexamethylene bisacetamide induced erythroleukemia differentiation.
    Prog Clin Biol Res. 1987;251:253-68 PMID: 3481077
  15. Characterization of mouse H3.3-like histone genes.
    Gene. 1987;59(1):29-39 PMID: 2449379
  16. Constitutive expression of a c-myb cDNA blocks Friend murine erythroleukemia cell differentiation.
    Mol Cell Biol. 1988 Feb;8(2):884-92 PMID: 2832742
  17. Probability that the commitment of murine erythroleukemia cell differentiation is determined by the c-myc level.
    J Mol Biol. 1988 Aug 20;202(4):779-86 PMID: 3172238
  18. Expression of a mouse replacement histone H3.3 gene with a highly conserved 3' noncoding region during SV40- and polyoma-induced Go to S-phase transition.
    Nucleic Acids Res. 1989 Apr 11;17(7):2449-61 PMID: 2470025
  19. Rapid induction of polyadenylated H1 histone mRNAs in mouse erythroleukemia cells is regulated by c-myc.
    Mol Cell Biol. 1989 Jun;9(6):2332-40 PMID: 2668731
  20. Different 3'-end processing produces two independently regulated mRNAs from a single H1 histone gene.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7002-6 PMID: 2780558
  21. Competition hybridization by "pre-saturation" of HeLa cell DNA.
    J Mol Biol. 1969 Sep 28;44(3):551-62 PMID: 5388124
  22. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide.
    Proc Natl Acad Sci U S A. 1971 Feb;68(2):378-82 PMID: 5277089
  23. Commitment to erythroid differentiation by friend erythroleukemia cells: a stochastic analysis.
    Cell. 1976 Oct;9(2):221-9 PMID: 975244
  24. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  25. Erythroleukemic differentiation.
    Annu Rev Biochem. 1978;47:419-48 PMID: 354501
  26. Inducer-mediated commitment of murine erythroleukemia cells to differentiation: a multistep process.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):471-5 PMID: 6952199
  27. Expression of c-myc changes during differentiation of mouse erythroleukaemia cells.
    Nature. 1984 Aug 16-22;310(5978):592-4 PMID: 6462247
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.12.2873
Published
1993-06-25
Pages
2873-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309673
Subset
IM
Grants
NCI NIH HHS · 2P30 CA 13330 · United States
NCI NIH HHS · CA 16368 · United States
External Links
PubMed source DOI: 10.1093/nar/21.12.2873 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2