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PMID: 8321217 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell cycle regulation of the c-Myc transcriptional activation domain.

Molecular and cellular biology ·Vol. 13 ·No. 7 ·1993-07-00 ·Pages 4125-36

Seth A, Gupta S, Davis RJ

Abstract

The product of the c-myc gene (c-Myc) is a sequence-specific DNA-binding protein that has previously been demonstrated to be required for cell cycle progression. Here we report that the c-Myc DNA binding site confers cell cycle regulation to a reporter gene in Chinese hamster ovary cells. The observed transactivation was biphasic with a small increase in G1 and a marked increase during the S-to-G2/M transition of the cell cycle. This cell cycle regulation of transactivation potential is accounted for, in part, by regulatory phosphorylation of the c-Myc transactivation domain. Together, these data demonstrate that c-Myc may have an important role in the progression of cells through both the G1 and G2 phases of the cell cycle.

Related Genes
c-myc
MeSH Terms
Animals Base Sequence Binding Sites CHO Cells Cell Cycle/genetics Cell Line Cricetinae DNA Gene Expression Regulation Genes, myc Kinetics Molecular Sequence Data Phosphorylation Serine/metabolism Transcription, Genetic Transcriptional Activation
Chemicals
Serine DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Seth A
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605.
Gupta S
Davis R J
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.7.4125-4136.1993
Published
1993-07-00
Pages
4125-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359962
Subset
IM
Grants
NCI NIH HHS · CA58396 · United States
NIGMS NIH HHS · GM37845 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.7.4125-4136.1993 PMC Full Text View journal details
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