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PMID: 1566084 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alternative forms of Max as enhancers or suppressors of Myc-ras cotransformation.

Science (New York, N.Y.) ·Vol. 256 ·No. 5055 ·1992-04-17 ·Pages 373-7

Mäkelä TP, Koskinen PJ, Västrik I, Alitalo K

Abstract

Max is a basic-helix-loop-helix-leucine zipper protein capable of forming sequence-specific DNA binding complexes with Myc proteins. An alternatively spliced messenger RNA has been identified that encodes a form of Max truncated at the COOH-terminus. This delta Max protein retained the ability to bind to the CACGTG motif in a complex with c-Myc but lacks the nuclear localization signal and the putative regulatory domain of Max. When tested in a myc-ras cotransformation assay in rat embryo fibroblasts, Max suppressed, whereas delta Max enhanced, transformation. Thus, the max gene may encode both a negative and a positive regulator of c-Myc function.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors Binding Sites Cell Nucleus/metabolism Cell Transformation, Neoplastic/drug effects,genetics DNA/chemistry,metabolism DNA-Binding Proteins/genetics,metabolism,pharmacology Fibroblasts Genes, myc Genes, ras Humans Immunosorbent Techniques Molecular Sequence Data Polymerase Chain Reaction Proto-Oncogene Proteins c-myc/genetics,metabolism RNA Splicing RNA, Messenger/genetics Rats Structure-Activity Relationship Transcription Factors Transfection

Chemicals

Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Basic-Leucine Zipper Transcription Factors DNA-Binding Proteins MAX protein, human Max protein, rat Myc associated factor X Proto-Oncogene Proteins c-myc RNA, Messenger Transcription Factors DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mäkelä T P

Department of Virology, University of Helsinki, Finland.

Koskinen P J

Västrik I

Alitalo K

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.256.5055.373

Published

1992-04-17

Pages

373-7

Language

English

Region

United States

NLM ID

0404511

Subset

Databases

GENBANK

X60287

External Links

PubMed source DOI: 10.1126/science.256.5055.373 View journal details

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Alternative forms of Max as enhancers or suppressors of Myc-ras cotransformation.

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