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PMID: 82935 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

Nucleic acids research ·Vol. 5 ·No. 11 ·1978-11-00 ·Pages 4055-64

Tomassini J, Roychoudhury R, Wu R, Roberts RJ

Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

MeSH Terms

Avian Myeloblastosis Virus/enzymology Base Sequence DNA DNA Restriction Enzymes Deoxyribonucleases Deoxyribonucleotides/analysis Klebsiella pneumoniae/enzymology Phosphoric Diester Hydrolases RNA-Directed DNA Polymerase

Chemicals

Deoxyribonucleotides DNA RNA-Directed DNA Polymerase Deoxyribonucleases DNA Restriction Enzymes Phosphoric Diester Hydrolases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Tomassini J

Roychoudhury R

Wu R

Roberts R J

References (13)

13 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/5.11.4055

Published

1978-11-00

Pages

4055-64

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342733

Subset

External Links

PubMed source DOI: 10.1093/nar/5.11.4055 PMC Full Text View journal details

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Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

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