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PMID: 265588 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 3 ·1977-03-00 ·Pages 966-70

Bahl CP, Wu R, Stawinsky J, Narang SA

Abstract

A number of specific duplex DNA sequences which correspond to varying lengths of the lactose operator region have been synthesized by a combination of chemical and enzymatic methods. Repressor binding studies on these synthetic duplex operator molecules show that all the nucleotides essential for full lactose operator-repressor interactions are included in a 17-nucleotide-long duplex DNA that constitutes the minimal recognition sequence for this DNA-protein interaction.

MeSH Terms
Base Sequence DNA/chemical synthesis DNA, Bacterial/metabolism Enzyme Repression Genes, Regulator Lactose/metabolism Operon Protein Binding Structure-Activity Relationship
Chemicals
DNA, Bacterial DNA Lactose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bahl C P
Wu R
Stawinsky J
Narang S A
References (23)
23 references, click to expand
  1. Chemical synthesis and sequence studies of deoxyribooligonucleotides which constitute the duplex sequence of the lactose operator of Escherichia coli.
    J Biol Chem. 1975 Jun 25;250(12):4592-600 PMID: 166998
  2. The use of arylsulfonyltriazoles for the synthesis of oligonucleotides by the triester approach.
    J Am Chem Soc. 1975 Dec 10;97(25):7332-7 PMID: 172538
  3. Structure of the lactose operator.
    Nature. 1976 Mar 25;260(5549):360-3 PMID: 768781
  4. Cloned synthetic lac operator DNA is biologically active.
    Nature. 1976 Oct 28;263(5580):744-8 PMID: 792711
  5. A reliable mapping method for sequence determination of oligodeoxyribonucleotides by mobility shift analysis.
    Anal Biochem. 1976 Jul;74(1):73-93 PMID: 962085
  6. A general method for inserting specific DNA sequences into cloning vehicles.
    Gene. 1976;1(1):81-92 PMID: 1052323
  7. Synthetic lac operator DNA is functional in vivo.
    Nature. 1976 Oct 28;263(5580):748-52 PMID: 1069185
  8. Genetic regulation: the Lac control region.
    Science. 1975 Jan 10;187(4171):27-35 PMID: 1088926
  9. The predicted secondary structure of the N-terminal sequence of the lac repressor and proposed models for its complexation to the lac operator.
    Biochemistry. 1975 Mar 11;14(5):1057-9 PMID: 1092326
  10. On the statistical significance of primary structural features found in DNA-protein interaction sites.
    Nucleic Acids Res. 1975 Mar;2(3):327-45 PMID: 1093137
  11. Chemical and enzymatic synthesis of lactose operator of Escherichia coli and its binding to lactose repressor.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):91-4 PMID: 1108022
  12. Improved triester approach for the synthesis of pentadecathymidylic acid.
    J Am Chem Soc. 1975 Dec 10;97(25):7327-32 PMID: 1194600
  13. Nucleotide sequence analysis of DNA. I. Partial sequence of the cohesive ends of bacteriophage lambda and 186 DNA.
    J Mol Biol. 1970 Aug;51(3):501-21 PMID: 4321727
  14. RNA-directed DNA synthesis and RNA tumor viruses.
    Adv Virus Res. 1972;17:129-86 PMID: 4348509
  15. Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase.
    Biochemistry. 1973 Dec 4;12(25):5045-50 PMID: 4366077
  16. Lac repressor. Specific proteolytic destruction of the NH 2 -terminal region and loss of the deoxyribonucleic acid-binding activity.
    J Biol Chem. 1973 Jan 10;248(1):110-21 PMID: 4571224
  17. The nucleotide sequence of the lac operator.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3581-4 PMID: 4587255
  18. Enzymatic breakage and joining of deoxyribonucleic acid. VI. Further purification and properties of polynucleotide ligase from Escherichia coli infected with bacteriophage T4.
    J Biol Chem. 1968 Sep 10;243(17):4543-55 PMID: 4879167
  19. Lac repressor-operator interaction. I. Equilibrium studies.
    J Mol Biol. 1970 Feb 28;48(1):67-83 PMID: 4915295
  20. Studies on polynucleotides, C. A novel joining reaction catalyzed by the T4-polynucleotide ligase.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1468-75 PMID: 5274471
  21. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.
    Nucleic Acids Res. 1974 Mar;1(3):331-53 PMID: 10793670
  22. Genetic regulatory mechanisms in the synthesis of proteins.
    J Mol Biol. 1961 Jun;3:318-56 PMID: 13718526
  23. Isolation of the lac repressor.
    Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8 PMID: 16591435
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.3.966
Published
1977-03-00
Pages
966-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430549
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.3.966 PMC Full Text View journal details
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