Home LiteratureArticle Details
PMID: 8289338 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The bovine papillomavirus type 1 (BPV1) replication protein E1 modulates transcriptional activation by interacting with BPV1 E2.

Journal of virology ·Vol. 68 ·No. 2 ·1994-02-00 ·Pages 1085-93

Le Moal MA, Yaniv M, Thierry F

Abstract

The study of bovine papillomavirus type 1 (BPV1) DNA replication has shown that E1 and E2 are the only viral proteins required for this process. Both E1 and E2 interact with the viral origin of replication (ori). The BPV1 E2 protein is also a well-characterized transcriptional regulator. We show in this report that E1 can modulate transcription by interactions with E2. At low concentrations, E1 enhanced the E2-mediated transactivation of heterologous promoters containing the BPV1 ori by promoting cooperative binding of both E1 and E2 to the DNA. In contrast, in the presence of excess E1, transactivation by E2 is repressed. This last process, however, does not require cooperative DNA binding of the two proteins. These results imply that the balance between these two distinct types of interaction is crucial both for control of replication and for early viral transcription.

MeSH Terms
Base Sequence Bovine papillomavirus 1/genetics Cell Compartmentation Cell Nucleus/metabolism DNA, Viral/metabolism DNA-Binding Proteins/metabolism Gene Expression Regulation, Viral Molecular Sequence Data Precipitin Tests Promoter Regions, Genetic/genetics Protein Binding Repressor Proteins/metabolism Thymidine Kinase/genetics Transcriptional Activation Viral Proteins/metabolism Virus Replication/genetics
Chemicals
DNA, Viral DNA-Binding Proteins E1 protein, Bovine papillomavirus E2 protein, Bovine papillomavirus Repressor Proteins Viral Proteins Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Le Moal M A
Unité des Virus Oncogènes, U1644 Centre National de la Recherche Scientifique, Institut Pasteur, Paris, France.
Yaniv M
Thierry F
References (40)
40 references, click to expand
  1. The bovine papillomavirus origin of replication requires a binding site for the E2 transcriptional activator.
    Proc Natl Acad Sci U S A. 1993 Feb 1;90(3):898-902 PMID: 8381536
  2. Viral E1 and E2 proteins support replication of homologous and heterologous papillomaviral origins.
    Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5799-803 PMID: 1321423
  3. Characterization of human papillomavirus type 11 E1 and E2 proteins expressed in insect cells.
    J Virol. 1993 May;67(5):2655-63 PMID: 8386271
  4. The acidic transcriptional activation domains of VP16 and p53 bind the cellular replication protein A and stimulate in vitro BPV-1 DNA replication.
    Cell. 1993 Jun 18;73(6):1207-21 PMID: 8390328
  5. The transactivator proteins VP16 and GAL4 bind replication factor A.
    Cell. 1993 Jun 18;73(6):1223-32 PMID: 8513504
  6. The human papillomavirus type 18 (HPV18) E2 gene product is a repressor of the HPV18 regulatory region in human keratinocytes.
    J Virol. 1989 Oct;63(10):4317-24 PMID: 2476572
  7. Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells.
    Nucleic Acids Res. 1989 Aug 11;17(15):6419 PMID: 2771659
  8. Repression of bovine papillomavirus type 1 transcription by the E1 replication protein.
    J Virol. 1993 Sep;67(9):5079-87 PMID: 8394436
  9. Mutational analysis of cis elements involved in E2 modulation of human papillomavirus type 16 P97 and type 18 P105 promoters.
    J Virol. 1990 Jun;64(6):2849-59 PMID: 2159546
  10. Cooperative activation of transcription by bovine papillomavirus type 1 E2 can occur over a large distance.
    Mol Cell Biol. 1990 Aug;10(8):4431-7 PMID: 2164642
  11. Distinct classes of transcriptional activating domains function by different mechanisms.
    Cell. 1990 Sep 21;62(6):1177-87 PMID: 2205398
  12. The 68-kilodalton E1 protein of bovine papillomavirus is a DNA binding phosphoprotein which associates with the E2 transcriptional activator in vitro.
    J Virol. 1991 Feb;65(2):649-56 PMID: 1846189
  13. Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator.
    Science. 1990 Dec 21;250(4988):1694-9 PMID: 2176744
  14. Cooperative binding of Drosophila heat shock factor to arrays of a conserved 5 bp unit.
    Cell. 1991 Feb 8;64(3):585-93 PMID: 1899357
  15. Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames.
    EMBO J. 1991 Feb;10(2):449-57 PMID: 1846806
  16. Cooperative binding of the E2 protein of bovine papillomavirus to adjacent E2-responsive sequences.
    J Virol. 1991 Apr;65(4):2124-30 PMID: 1848322
  17. Direct interaction between Sp1 and the BPV enhancer E2 protein mediates synergistic activation of transcription.
    Cell. 1991 May 3;65(3):493-505 PMID: 1850324
  18. Functional analysis of E2-mediated repression of the HPV18 P105 promoter.
    New Biol. 1991 Jan;3(1):90-100 PMID: 1645591
  19. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  20. Aphidicolin prevents mitotic cell division by interfering with the activity of DNA polymerase-alpha.
    Nature. 1978 Oct 5;275(5679):458-60 PMID: 692726
  21. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  22. A common function for polyoma virus large-T and papillomavirus E1 proteins?
    Nature. 1984 Sep 20-26;311(5983):276-9 PMID: 6090931
  23. Transactivation of a bovine papilloma virus transcriptional regulatory element by the E2 gene product.
    Cell. 1985 Aug;42(1):183-91 PMID: 2990724
  24. Bovine papillomavirus transcriptional regulation: localization of the E2-responsive elements of the long control region.
    J Virol. 1987 Jul;61(7):2128-37 PMID: 3035214
  25. The BPV1-E2 trans-acting protein can be either an activator or a repressor of the HPV18 regulatory region.
    EMBO J. 1987 Nov;6(11):3391-7 PMID: 2828029
  26. Cooperative DNA binding of the yeast transcriptional activator GAL4.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):382-6 PMID: 3124106
  27. The specific DNA recognition sequence of the bovine papillomavirus E2 protein is an E2-dependent enhancer.
    EMBO J. 1988 Feb;7(2):525-31 PMID: 2835231
  28. Negative effect of the transcriptional activator GAL4.
    Nature. 1988 Aug 25;334(6184):721-4 PMID: 3412449
  29. Bovine papillomavirus type 1 E1 replication-defective mutants are altered in their transcriptional regulation.
    J Virol. 1988 Nov;62(11):4009-15 PMID: 2845119
  30. A dimer of BPV-1 E2 containing a protease resistant core interacts with its DNA target.
    EMBO J. 1988 Dec 1;7(12):3807-16 PMID: 2850174
  31. Identification of bovine papillomavirus E1 mutants with increased transforming and transcriptional activity.
    J Virol. 1989 Apr;63(4):1775-82 PMID: 2538656
  32. Specific recognition nucleotides and their DNA context determine the affinity of E2 protein for 17 binding sites in the BPV-1 genome.
    Genes Dev. 1989 Apr;3(4):510-26 PMID: 2542129
  33. Formation of the complex of bovine papillomavirus E1 and E2 proteins is modulated by E2 phosphorylation and depends upon sequences within the carboxyl terminus of E1.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6363-7 PMID: 1648739
  34. Two DNA-bound E2 dimers are required for strong transcriptional activation and for cooperation with cellular factors in most cells.
    New Biol. 1991 May;3(5):498-509 PMID: 1653009
  35. The functional BPV-1 E2 trans-activating protein can act as a repressor by preventing formation of the initiation complex.
    Genes Dev. 1991 Sep;5(9):1657-71 PMID: 1653173
  36. A bovine papillomavirus E1-related protein binds specifically to bovine papillomavirus DNA.
    J Virol. 1991 Oct;65(10):5314-22 PMID: 1654443
  37. Several different upstream promoter elements can potentiate transactivation by the BPV-1 E2 protein.
    EMBO J. 1991 Oct;10(10):2931-40 PMID: 1655407
  38. Activation of BPV-1 replication in vitro by the transcription factor E2.
    Nature. 1991 Oct 17;353(6345):628-32 PMID: 1656277
  39. Identification of the origin of replication of bovine papillomavirus and characterization of the viral origin recognition factor E1.
    EMBO J. 1991 Dec;10(13):4321-9 PMID: 1661672
  40. Bovine papilloma virus (BPV)-encoded E2 protein enhances binding of E1 protein to the BPV replication origin.
    Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):2865-9 PMID: 8385347
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.2.1085-1093.1994
Published
1994-02-00
Pages
1085-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC236547
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.68.2.1085-1093.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2