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PMID: 8265616 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Multiple motifs regulate the B-cell-specific promoter of the B29 gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 24 ·1993-12-15 ·Pages 11723-7

Omori SA, Wall R

Abstract

The B-cell-specific B29 and mb1 genes code for covalently linked proteins (B29 or Ig beta and mb1 or Ig alpha, respectively) associated with membrane immunoglobulins in the antigen receptor complex on B cells. We have functionally analyzed the upstream region of the B29 gene and have identified a 164-bp region which comprises the minimal promoter responsible for B-cell-specific transcription. Linker scanning mutagenesis of this minimal promoter has established that both the previously identified octamer motif and a DNA motif that binds an unknown protein factor are critical for B29 gene expression in a pre-B-cell and B-cell line. Further mutations showed that binding motifs for Ets, microB/LyF1, and Sp1 also significantly contributed to the overall activity of the minimal B29 promoter. However, the relative contribution of certain motifs to promoter activity was different in a pre-B versus a B-cell line. The microB/LyF1 motif was necessary for full promoter activity in the pre-B cells but was not required in the B cells.

Related Genes
B29 mb1
MeSH Terms
Animals Antigens, CD B-Lymphocytes/metabolism Base Sequence CD79 Antigens Cell Line Cell Nucleus/metabolism Chloramphenicol O-Acetyltransferase DNA-Binding Proteins/metabolism Deoxyribonuclease I Gene Expression Regulation Membrane Glycoproteins/biosynthesis Molecular Sequence Data Mutagenesis, Insertional Phosphoproteins/biosynthesis Promoter Regions, Genetic Transcription, Genetic Transfection
Chemicals
Antigens, CD CD79 Antigens DNA-Binding Proteins Membrane Glycoproteins Phosphoproteins Chloramphenicol O-Acetyltransferase Deoxyribonuclease I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Omori S A
Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90024.
Wall R
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48 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.24.11723
Published
1993-12-15
Pages
11723-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48056
Subset
IM
Grants
NCI NIH HHS · CA12800 · United States
NIGMS NIH HHS · GM07104 · United States
NIGMS NIH HHS · GM40185 · United States
Databases
GENBANK
M26184
External Links
PubMed source DOI: 10.1073/pnas.90.24.11723 PMC Full Text View journal details
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