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PMID: 8223450 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

DNA curvature influences the internal motions of supercoiled DNA.

The EMBO journal ·Vol. 12 ·No. 11 ·1993-11-00 ·Pages 4407-12

Kremer W, Klenin K, Diekmann S, Langowski J

Abstract

We present evidence that short curved DNA segments can act as mediators for the ordering of large domains in superhelical DNA. Using a non-invasive solution method (dynamic light scattering), we investigated the effect of permanently curved inserts on the solution structure and on the internal motions of superhelical plasmid DNA. We find that the dynamics of superhelical DNA are strongly influenced by sequence- or protein-induced bending: in superhelical plasmids containing curved inserts the amplitude of the internal motion is lower than that of non-curved controls. Furthermore, the relative arrangement of curved sequences in the plasmids can influence the overall shape of the superhelical DNA. On linearized forms of the plasmids, these effects are not observed.

MeSH Terms
Base Sequence Computer Simulation DNA, Bacterial/chemistry DNA, Superhelical/chemistry Diffusion Molecular Sequence Data Monte Carlo Method Motion Nucleic Acid Conformation Plasmids/chemistry Restriction Mapping
Chemicals
DNA, Bacterial DNA, Superhelical
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kremer W
EMBL, Grenoble Outstation, France.
Klenin K
Diekmann S
Langowski J
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30 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-11-00
Pages
4407-12
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413738
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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