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PMID: 2323339 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Electron microscopy mapping of pBR322 DNA curvature. Comparison with theoretical models.

The EMBO journal ·Vol. 9 ·No. 4 ·1990-04-00 ·Pages 1289-98

Muzard G, Théveny B, Révet B

Abstract

A map of local curvature of the pBR322 DNA has been established by electron microscopy analysis of linearized plasmid molecules. To determine their polarity these molecules are one end labelled with an avidin-ferritin-biotin complex and the images are digitized. Local curvature is calculated from two mathematical treatments of the DNA trajectory and expressed in term of a mean dinucleotide wedge angle. Eight regions of curvature are distinguished. The four main regions of curvature have a high content of phased AA runs. The experimental curvature map is compared to theoretical maps of curvature obtained from four available models for DNA curvature.

MeSH Terms
Base Composition DNA, Bacterial/ultrastructure DNA, Superhelical/ultrastructure Mathematics Microscopy, Electron/methods Models, Theoretical Nucleic Acid Conformation Plasmids Restriction Mapping
Chemicals
DNA, Bacterial DNA, Superhelical
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Muzard G
Laboratoire de Pharmacologie Moléculaire, (CNRS UA 147) Institut Gustave-Roussy, Villejuif, France.
Théveny B
Révet B
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-04-00
Pages
1289-98
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551807
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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