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PMID: 8223443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TCR beta and TCR alpha gene enhancers confer tissue- and stage-specificity on V(D)J recombination events.

The EMBO journal ·Vol. 12 ·No. 11 ·1993-11-00 ·Pages 4335-46

Capone M, Watrin F, Fernex C, Horvat B, Krippl B, Wu L, Scollay R, Ferrier P

Abstract

We describe transgenic mice carrying germline variable gene segments associated with either the T cell receptor (TCR) beta or alpha gene enhancers (E beta or E alpha). Transgenic constructs underwent high rates of site-specific rearrangements predominantly in T cells from independent mice. Rearrangements of the E beta-containing transgenes began at different stages of T cell differentiation in embryonic and adult thymus than did the E alpha-containing ones, with a pattern superimposable upon the patterns of TCR beta or TCR alpha gene expression, respectively. We demonstrate that sequences within the TCR beta and TCR alpha gene enhancers confer tissue- and stage-specificity upon the V(D)J recombination events affecting adjacent gene segments. The patterns of transgene expression also gave information on developmental events and lineage relationships (gamma delta versus alpha beta) during T cell development.

MeSH Terms
Aging Animals B-Lymphocytes/immunology DNA Nucleotidyltransferases/metabolism Enhancer Elements, Genetic/genetics Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor Gene Rearrangement, beta-Chain T-Cell Antigen Receptor Hematopoietic Stem Cells/immunology Lymph Nodes/immunology Mice Mice, Inbred C57BL Mice, Inbred CBA Mice, Transgenic Receptors, Antigen, T-Cell, alpha-beta/genetics Recombination, Genetic T-Lymphocyte Subsets/immunology T-Lymphocytes/immunology Thymus Gland/embryology,immunology VDJ Recombinases
Chemicals
Receptors, Antigen, T-Cell, alpha-beta DNA Nucleotidyltransferases VDJ Recombinases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Capone M
Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, France.
Watrin F
Fernex C
Horvat B
Krippl B
Wu L
Scollay R
Ferrier P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-11-00
Pages
4335-46
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413730
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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