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PMID: 8221925 Published · ppublish English Journal Article Review

MCB elements and the regulation of DNA replication genes in yeast.

Current genetics ·Vol. 24 ·No. 3 ·1993-09-00 ·Pages 185-92

McIntosh EM

Abstract

In eukaryotic organisms, genes involved in DNA replication are often subject to some form of cell cycle control. In the yeast Saccharomyces cerevisiae, most of the DNA replication genes that have been characterized to date are regulated at the transcriptional level during G1 to S phase transition. A cis-acting element termed the MluI cell cycle box (or MCB) conveys this pattern of regulation and is common among more than 20 genes involved in DNA synthesis and repair. Recent findings indicate that the MCB element is well conserved among fungi and may play a role in controlling entry into the cell division cycle. It is evident from studies in higher systems, however, that transcriptional regulation is not the only form of control that governs the cell-cycle-dependent expression of DNA replication genes. Moreover, it is unclear why this general pattern of regulation exists for so many of these genes in various eukaryotic systems. This review summarizes recent studies of the MCB element in yeast and briefly discusses the purpose of regulating DNA replication genes in the eukaryotic cell cycle.

MeSH Terms
Bacterial Proteins Base Sequence Cell Cycle/genetics DNA Replication/genetics DNA, Fungal/genetics Deoxyribonucleases, Type II Site-Specific/metabolism Molecular Sequence Data Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics
Chemicals
Bacterial Proteins DNA, Fungal endodeoxyribonuclease MluI Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
McIntosh E M
Department of Biology, York University, Toronto, Canada.
References (76)
76 references, click to expand
  1. The identification of a second cell cycle control on the HO promoter in yeast: cell cycle regulation of SW15 nuclear entry.
    Cell. 1990 Aug 24;62(4):631-47 PMID: 2167175
  2. The periodically expressed TMP1 gene of Saccharomyces cerevisiae is subject to START-dependent and START-independent regulation.
    J Biol Chem. 1991 Sep 5;266(25):16808-12 PMID: 1885607
  3. Cell cycle-dependent phosphorylation of human DNA polymerase alpha.
    J Biol Chem. 1991 Apr 25;266(12):7893-903 PMID: 1902230
  4. Changes in a SWI4,6-DNA-binding complex occur at the time of HO gene activation in yeast.
    Genes Dev. 1991 Nov;5(11):2000-13 PMID: 1936990
  5. The c-myb proto-oncogene product binds to but does not activate the promoter of the DNA polymerase alpha gene.
    Oncogene. 1992 Oct;7(10):1999-2006 PMID: 1408140
  6. The CDC8 transcript is cell cycle regulated in yeast and is expressed coordinately with CDC9 and CDC21 at a point preceding histone transcription.
    Exp Cell Res. 1987 Jul;171(1):223-31 PMID: 3305044
  7. Regulation of cell cycle-dependent gene expression in yeast.
    J Biol Chem. 1990 Aug 25;265(24):14057-60 PMID: 2201678
  8. A family of cyclin homologs that control the G1 phase in yeast.
    Proc Natl Acad Sci U S A. 1989 Aug;86(16):6255-9 PMID: 2569741
  9. Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo.
    Mol Cell Biol. 1992 Dec;12(12):5724-35 PMID: 1448101
  10. Interaction of the yeast Swi4 and Swi6 cell cycle regulatory proteins in vitro.
    Proc Natl Acad Sci U S A. 1992 Dec 15;89(24):11852-6 PMID: 1465410
  11. Gene expression of human DNA polymerase alpha during cell proliferation and the cell cycle.
    Mol Cell Biol. 1988 Nov;8(11):5016-25 PMID: 3211134
  12. Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase.
    Genes Dev. 1990 May;4(5):740-51 PMID: 2199320
  13. Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae.
    Mol Cell Biol. 1991 Jan;11(1):329-37 PMID: 1986229
  14. Periodic events in the cell cycle.
    Curr Opin Cell Biol. 1990 Apr;2(2):274-9 PMID: 2194527
  15. Herpes simplex virus type 1 dUTPase mutants are attenuated for neurovirulence, neuroinvasiveness, and reactivation from latency.
    J Virol. 1992 Nov;66(11):6706-13 PMID: 1328686
  16. Retinoblastoma. A transcriptional tryst.
    Nature. 1991 Jul 18;352(6332):189-90 PMID: 1857408
  17. An essential G1 function for cyclin-like proteins in yeast.
    Cell. 1989 Dec 22;59(6):1127-33 PMID: 2574633
  18. Cyclin E/cdk2 and cyclin A/cdk2 kinases associate with p107 and E2F in a temporally distinct manner.
    Genes Dev. 1992 Oct;6(10):1874-85 PMID: 1398067
  19. Cell cycle regulation of the human cdc2 gene.
    EMBO J. 1992 May;11(5):1797-804 PMID: 1582409
  20. Isolation and characterization of the Schizosaccharomyces pombe rad3 gene, involved in the DNA damage and DNA synthesis checkpoints.
    Gene. 1992 Sep 21;119(1):83-9 PMID: 1398093
  21. Positive cis-acting regulatory sequences mediate proper control of POL1 transcription in Saccharomyces cerevisiae.
    Curr Genet. 1992 Mar;21(3):183-9 PMID: 1563043
  22. Human dUTP pyrophosphatase: cDNA sequence and potential biological importance of the enzyme.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8020-4 PMID: 1325640
  23. CLB5: a novel B cyclin from budding yeast with a role in S phase.
    Genes Dev. 1992 Sep;6(9):1695-706 PMID: 1387626
  24. G1-specific cyclins of S. cerevisiae: cell cycle periodicity, regulation by mating pheromone, and association with the p34CDC28 protein kinase.
    Cell. 1990 Jul 27;62(2):225-37 PMID: 2142620
  25. A new family of yeast nuclear pore complex proteins.
    J Cell Biol. 1992 Nov;119(4):705-23 PMID: 1385442
  26. Genomic structure and chromosomal localization of the human deoxycytidine kinase gene.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):431-4 PMID: 8421671
  27. Structural and functional analysis of the human thymidylate synthase gene.
    J Biol Chem. 1990 Nov 25;265(33):20277-84 PMID: 2243092
  28. Direct induction of G1-specific transcripts following reactivation of the Cdc28 kinase in the absence of de novo protein synthesis.
    Genes Dev. 1992 Apr;6(4):557-67 PMID: 1313770
  29. Cell-cycle-regulated phosphorylation of DNA replication factor A from human and yeast cells.
    Genes Dev. 1990 Jun;4(6):968-77 PMID: 2200738
  30. Growth-rate-dependent regulation of the expression and inactivation of thymidylate synthase in Saccharomyces cerevisiae.
    J Bacteriol. 1986 Dec;168(3):1336-42 PMID: 3536872
  31. A central role for SWI6 in modulating cell cycle Start-specific transcription in yeast.
    Nature. 1992 Jun 11;357(6378):508-13 PMID: 1608451
  32. Identification of a DNA binding factor involved in cell-cycle control of the yeast HO gene.
    Cell. 1989 Apr 7;57(1):21-9 PMID: 2649246
  33. The yeast DNA polymerase I transcript is regulated in both the mitotic cell cycle and in meiosis and is also induced after DNA damage.
    Nucleic Acids Res. 1987 Jul 10;15(13):5017-30 PMID: 3299263
  34. Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constitutive transcription in yeast.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8419-23 PMID: 3909145
  35. Transcriptional repression of the E2-containing promoters EIIaE, c-myc, and RB1 by the product of the RB1 gene.
    Mol Cell Biol. 1992 Aug;12(8):3431-8 PMID: 1385853
  36. CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Dec;12(12):5736-47 PMID: 1341195
  37. Anatomy of a transcription factor important for the start of the cell cycle in Saccharomyces cerevisiae.
    Nature. 1992 Aug 13;358(6387):593-7 PMID: 1386897
  38. Periodic transcription as a means of regulating gene expression during the cell cycle: contrasting modes of expression of DNA ligase genes in budding and fission yeast.
    EMBO J. 1986 Jul;5(7):1705-9 PMID: 3527694
  39. Cell cycle regulation of thymidine kinase: residues near the carboxyl terminus are essential for the specific degradation of the enzyme at mitosis.
    Mol Cell Biol. 1991 May;11(5):2538-46 PMID: 1708095
  40. A role for the adenovirus inducible E2F transcription factor in a proliferation dependent signal transduction pathway.
    EMBO J. 1990 Jul;9(7):2179-84 PMID: 2141565
  41. SWI6 protein is required for transcription of the periodically expressed DNA synthesis genes in budding yeast.
    Nature. 1992 Jun 11;357(6378):505-8 PMID: 1608450
  42. Histone H3 transcription in Saccharomyces cerevisiae is controlled by multiple cell cycle activation sites and a constitutive negative regulatory element.
    Mol Cell Biol. 1992 Dec;12(12):5455-63 PMID: 1448078
  43. Cell cycle-dependent expression of thymidylate synthase in Saccharomyces cerevisiae.
    Mol Cell Biol. 1984 Dec;4(12):2858-64 PMID: 6396509
  44. The nucleotide sequence of the fission yeast DNA topoisomerase II gene: structural and functional relationships to other DNA topoisomerases.
    EMBO J. 1986 Sep;5(9):2355-61 PMID: 3023070
  45. The enzymology of poxvirus DNA replication.
    Curr Top Microbiol Immunol. 1990;163:93-123 PMID: 2242683
  46. Identification of an RNA binding site for human thymidylate synthase.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):517-21 PMID: 8421684
  47. Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factor.
    Nature. 1991 Mar 21;350(6315):247-50 PMID: 2005980
  48. A cell cycle analysis of growth-related genes expressed during T lymphocyte maturation.
    J Cell Biol. 1990 Dec;111(6 Pt 1):2693-701 PMID: 2277081
  49. The regulation of histone synthesis in the cell cycle.
    Annu Rev Biochem. 1991;60:827-61 PMID: 1883210
  50. The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton.
    J Cell Biol. 1992 Mar;116(6):1319-32 PMID: 1541631
  51. The expression in meiosis of genes which are transcribed periodically in the mitotic cell cycle of budding yeast.
    Exp Cell Res. 1986 Aug;165(2):541-9 PMID: 3522259
  52. Ribonucleotide reductase: regulation, regulation, regulation.
    Trends Biochem Sci. 1992 Mar;17(3):119-23 PMID: 1412696
  53. Activation of mammalian DNA ligase I through phosphorylation by casein kinase II.
    EMBO J. 1992 Aug;11(8):2925-33 PMID: 1639065
  54. Cell cycle control of the yeast HO gene: cis- and trans-acting regulators.
    Cell. 1987 Feb 13;48(3):389-97 PMID: 3542227
  55. Transcriptional regulation in the eukaryotic cell cycle.
    Trends Biochem Sci. 1991 Nov;16(11):430-5 PMID: 1776174
  56. Cloning the REC1 gene of Ustilago maydis.
    Curr Genet. 1991 Jul;20(1-2):145-50 PMID: 1934111
  57. Similarity between cell-cycle genes of budding yeast and fission yeast and the Notch gene of Drosophila.
    Nature. 1987 Oct 15-21;329(6140):651-4 PMID: 2821408
  58. Analysis of the SWI4/SWI6 protein complex, which directs G1/S-specific transcription in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Feb;13(2):1069-77 PMID: 8423776
  59. Activation of the mouse proliferating cell nuclear antigen gene promoter by adenovirus type 12 E1A proteins.
    Jpn J Cancer Res. 1992 Jun;83(6):609-17 PMID: 1353754
  60. The yeast DNA ligase gene CDC9 is controlled by six orientation specific upstream activating sequences that respond to cellular proliferation but which alone cannot mediate cell cycle regulation.
    Nucleic Acids Res. 1991 Jan 25;19(2):359-64 PMID: 1901644
  61. Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7155-9 PMID: 1871128
  62. Transcriptional activation of CLN1, CLN2, and a putative new G1 cyclin (HCS26) by SWI4, a positive regulator of G1-specific transcription.
    Cell. 1991 Sep 6;66(5):1015-26 PMID: 1832336
  63. Regulation of human thymidine kinase during the cell cycle.
    J Biol Chem. 1988 Jun 15;263(17):8350-8 PMID: 3372530
  64. The WHI1+ gene of Saccharomyces cerevisiae tethers cell division to cell size and is a cyclin homolog.
    EMBO J. 1988 Dec 20;7(13):4335-46 PMID: 2907481
  65. Transcriptional regulation of the cell cycle-dependent thymidylate synthase gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4616-24 PMID: 3062362
  66. Identification of a protein-binding site in the promoter of the human thymidine kinase gene required for the G1-S-regulated transcription.
    J Biol Chem. 1992 Feb 5;267(4):2723-7 PMID: 1370831
  67. Independent binding of the retinoblastoma protein and p107 to the transcription factor E2F.
    Nature. 1992 Jan 9;355(6356):176-9 PMID: 1530885
  68. The M1 subunit of rat liver ribonucleotide reductase appears to be modified by ubiquitination.
    Biochem Cell Biol. 1992 Mar-Apr;70(3-4):215-23 PMID: 1325159
  69. DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4675-84 PMID: 3062366
  70. Transcription of genes encoding enzymes involved in DNA synthesis during the cell cycle of Saccharomyces cerevisiae.
    Mol Gen Genet. 1986 Sep;204(3):363-6 PMID: 3020375
  71. The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast.
    Cell. 1991 Sep 6;66(5):995-1013 PMID: 1832338
  72. Regulation of the yeast DNA replication genes through the Mlu I cell cycle box is dependent on SWI6.
    Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9479-83 PMID: 1409658
  73. Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome.
    Mol Cell Biol. 1992 Sep;12(9):3807-18 PMID: 1324406
  74. Control of DNA synthesis genes in fission yeast by the cell-cycle gene cdc10+.
    Nature. 1992 Jan 30;355(6359):449-53 PMID: 1734281
  75. Cell cycle control of DNA synthesis in budding yeast.
    Nucleic Acids Res. 1992 May 25;20(10):2403-10 PMID: 1598198
  76. Retinoblastoma protein switches the E2F site from positive to negative element.
    Nature. 1992 Jul 16;358(6383):259-61 PMID: 1321348
Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00351790
Published
1993-09-00
Pages
185-92
Language
English
Region
United States
NLM ID
8004904
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00351790 View journal details
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