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PMID: 1871128 Published · ppublish English Journal Article

Identification and purification of a factor that binds to the Mlu I cell cycle box of yeast DNA replication genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 16 ·1991-08-15 ·Pages 7155-9

Verma R, Patapoutian A, Gordon CB, Campbell JL

Abstract

In Saccharomyces cerevisiae, the genes encoding at least 10 enzymes involved in DNA replication are periodically expressed in the late G1 and S phases of the cell cycle. All of these genes have one copy or more of the sequence ACGCGT, which conforms to the recognition site for the Mlu I restriction endonuclease. For the CDC21, CDC9, and POL1 genes, the Mlu I site has been shown to be absolutely required for periodic transcription. Using nuclear extracts fractionated by conventional and oligonucleotide affinity chromatography, we have purified a 17-kDa protein that recognizes the Mlu I motif. Synthetic oligonucleotides containing mutated Mlu I sites do not bind the protein. In contrast, synthetic oligonucleotides derived from the CDC2, CDC6, and CDC21 genes, which are expressed with the same timing as POL1, bind purified protein efficiently.

MeSH Terms
Bacterial Proteins Base Sequence Carrier Proteins/isolation & purification,metabolism Cell Cycle/genetics Cell Nucleus/physiology DNA Polymerase I/genetics DNA Replication Deoxyribonuclease I Deoxyribonucleases, Type II Site-Specific Fungal Proteins/genetics Genes, Fungal Molecular Sequence Data Oligonucleotide Probes Restriction Mapping Saccharomyces cerevisiae/cytology,genetics,metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins Fungal Proteins Oligonucleotide Probes DNA Polymerase I endodeoxyribonuclease MluI Deoxyribonuclease I Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Verma R
Braun Laboratories, California Institute of Technology, Pasaden 91125.
Patapoutian A
Gordon C B
Campbell J L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.16.7155
Published
1991-08-15
Pages
7155-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52252
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.16.7155 PMC Full Text View journal details
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