Cloning the REC1 gene of Ustilago maydis.

Current genetics ·Vol. 20 ·No. 1-2 ·1991-07-00 ·Pages 145-50

Holden DW, Spanos A, Kanuga N, Banks GR

Abstract

The REC1 gene of Ustilago maydis plays a key role in homologous recombination and the repair of damaged DNA. In order to understand the nature and functions of the gene product, the gene has been cloned by functional complementation. A 3.8 kb cloned fragment complements the pleiotropic mitotic phenotype of different rec1 alleles. It does not complement the UV sensitivity of two other sensitive mutants. Disruption of the chromosomal copy of the 1.566 kb open reading frame within this fragment reproduces the rec1 pleiotropic phenotype. Furthermore, in diploids this disrupted reading frame is unable to complement previously characterised rec1 alleles.

Related Genes

REC1

MeSH Terms

Blotting, Southern Cloning, Molecular Cosmids DNA, Fungal Genes, Fungal Genetic Complementation Test Genomic Library Mutation Recombination, Genetic Restriction Mapping Ultraviolet Rays Ustilago/genetics,radiation effects

Chemicals

DNA, Fungal

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Holden D W

Genetics Division, National Institute for Medical Research, London, England.

Spanos A

Kanuga N

Banks G R

References (25)

25 references, click to expand

Heteroduplex formation and polarity during strand transfer promoted by Ustilago rec 1 protein.
Cell. 1983 Jul;33(3):857-64 PMID: 6871997
Homologous pairing of DNA molecules promoted by a protein from Ustilago.
Cell. 1982 Jun;29(2):367-74 PMID: 6214313
Nuclear location signal-mediated protein transport.
Biochim Biophys Acta. 1989 Aug 14;1008(3):263-80 PMID: 2667641
Isolation of the REC2 gene controlling recombination in Ustilago maydis.
Gene. 1990 Dec 15;96(2):285-8 PMID: 2269439
Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae.
Mol Cell Biol. 1991 Jan;11(1):329-37 PMID: 1986229
Cloning and disruption of Ustilago maydis genes.
Mol Cell Biol. 1989 Sep;9(9):4052-5 PMID: 2779577
Eukaryotic transcription factors.
Biochem J. 1990 Sep 1;270(2):281-9 PMID: 2119171
Genetic characterization of rec-1, a mutant of Ustilago maydis defective in repair and recombination.
Genet Res. 1976 Jun;27(3):413-53 PMID: 1001898
Further evidence for an inducible recombination repair system in Ustilago maydis.
Mutat Res. 1975 Jul;29(1):149-53 PMID: 1152856
Mutation in a heat-regulated hsp70 gene of Ustilago maydis.
EMBO J. 1989 Jul;8(7):1927-34 PMID: 2792075
Isolation of the REC1 gene controlling recombination in Ustilago maydis.
Gene. 1989 Dec 28;85(2):335-41 PMID: 2628171
Coordination of expression of DNA synthesis genes in budding yeast by a cell-cycle regulated trans factor.
Nature. 1991 Mar 21;350(6315):247-50 PMID: 2005980
Isolation and characterization of an autonomously replicating sequence from Ustilago maydis.
Mol Cell Biol. 1988 Sep;8(9):3703-9 PMID: 2851726
General method for cloning Neurospora crassa nuclear genes by complementation of mutants.
Mol Cell Biol. 1985 Sep;5(9):2272-8 PMID: 2942762
Gene transfer system for the phytopathogenic fungus Ustilago maydis.
Proc Natl Acad Sci U S A. 1988 Feb;85(3):865-9 PMID: 2829206
Radiation sensitive mutants of Ustilago maydis.
Mutat Res. 1965 Dec;2(6):557-9 PMID: 5878282
Inducible DNA repair in Ustilago maydis.
Mol Gen Genet. 1982;185(2):245-50 PMID: 6953306
Biochemical characterization of rec1 mutants and the genetic control of recombination in Ustilago maydis.
Cold Spring Harb Symp Quant Biol. 1984;49:669-73 PMID: 6597760
Cloning of the PYR3 gene of Ustilago maydis and its use in DNA transformation.
Mol Cell Biol. 1988 Dec;8(12):5417-24 PMID: 2907604
Altered recombination frequencies in radiation sensitivie strains of Ustilago.
Mutat Res. 1967 May-Jun;4(3):275-88 PMID: 6059022
Biochemical measure of the time and frequency of radiation-induced allelic recombination in Ustilago.
Nat New Biol. 1971 Aug 25;232(34):233-6 PMID: 5286192
Nucleotide sequence of the REC1 gene of Ustilago maydis.
Nucleic Acids Res. 1989 Dec 25;17(24):10489 PMID: 2602156
Zinc fingers, zinc clusters, and zinc twists in DNA-binding protein domains.
Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):999-1003 PMID: 1846973
Repair of double-strand breaks and lethal damage in DNA of Ustilago maydis.
Genet Res. 1980 Jun;35(3):291-307 PMID: 7439684
Analysis of ferrichrome biosynthesis in the phytopathogenic fungus Ustilago maydis: cloning of an ornithine-N5-oxygenase gene.
J Bacteriol. 1989 May;171(5):2811-8 PMID: 2523381

Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00312777

Published

1991-07-00

Pages

145-50

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00312777 View journal details

Analysis Services

Cloning the REC1 gene of Ustilago maydis.

Contact

WeChat Official Account