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PMID: 8182133 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Expression of alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein and scavenger receptor in human atherosclerotic lesions.

The Journal of clinical investigation ·Vol. 93 ·No. 5 ·1994-05-00 ·Pages 2014-21

Luoma J, Hiltunen T, Särkioja T, Moestrup SK, Gliemann J, Kodama T, Nikkari T, Ylä-Herttuala S

Abstract

Macrophage- and smooth muscle cell (SMC)-derived foam cells are typical constituents of human atherosclerotic lesions. At least three receptor systems have been characterized that could be involved in the development of foam cells: alpha 2-macroglobulin receptor/LDL receptor-related protein (alpha 2 MR/LRP), scavenger receptor, and LDL receptor. We studied the expression of these receptors in human atherosclerotic lesions with in situ hybridization and immunocytochemistry. An abundant expression of alpha 2MR/LRP mRNA and protein was found in SMC and macrophages in both early and advanced lesions in human aortas. alpha 2MR/LRP was also present in SMC in normal aortas. Scavenger receptor mRNA and protein were expressed in lesion macrophages but no expression was found in lesion SMC. LDL receptor was absent from the lesion area but was expressed in some aortas in medial SMC located near the adventitial border. The results demonstrate that (a) alpha 2MR/LRP is, so far, the only lipoprotein receptor expressed in lesions SMC in vivo; (b) scavenger receptors are expressed only in lesion macrophages; and (c) both receptors may play important roles in the development of human atherosclerotic lesions.

MeSH Terms
Adult Aged Aorta/chemistry,cytology Arteriosclerosis/metabolism Female Humans In Situ Hybridization Low Density Lipoprotein Receptor-Related Protein-1 Macrophages/chemistry Male Membrane Proteins Middle Aged Muscle, Smooth, Vascular/chemistry RNA, Messenger/isolation & purification Receptors, Immunologic/genetics,isolation & purification Receptors, Lipoprotein Receptors, Scavenger Scavenger Receptors, Class B
Chemicals
Low Density Lipoprotein Receptor-Related Protein-1 Membrane Proteins RNA, Messenger Receptors, Immunologic Receptors, Lipoprotein Receptors, Scavenger Scarb1 protein, mouse Scavenger Receptors, Class B
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Luoma J
Department of Biomedical Sciences, University of Tampere, Finland.
Hiltunen T
Särkioja T
Moestrup S K
Gliemann J
Kodama T
Nikkari T
Ylä-Herttuala S
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117195
Published
1994-05-00
Pages
2014-21
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC294312
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI117195 PMC Full Text View journal details
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