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PMID: 1423505 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Distribution of the alpha 2-macroglobulin receptor/low density lipoprotein receptor-related protein in human tissues.

Cell and tissue research ·Vol. 269 ·No. 3 ·1992-09-00 ·Pages 375-82

Moestrup SK, Gliemann J, Pallesen G

Abstract

The hepatic alpha 2-macroglobulin receptor (alpha 2MR)/low density lipoprotein receptor-related protein (LRP) binds and endocytoses alpha 2-macroglobulin-proteinase complexes in plasma. In addition, it binds lipoproteins, a novel 40 kDa protein, and complexes between plasminogen activators and plasminogen activator inhibitor type-1. This study shows, for the first time, the tissue distribution of alpha 2MR/LRP as determined by immunohistochemistry with specific monoclonal antibodies. The analysis revealed alpha 2MR/LRP-expression in a restricted spectrum of cell types, including neurons and astrocytes in the central nervous system, epithelial cells of the gastrointestinal tract, smooth muscle cells, fibroblasts, Leydig cells in testis, granulosa cells in ovary, and dendritic interstitial cells of kidney. Monocyte-derived cells displayed marked alpha 2MR/LRP expression in the phagocytes of liver, lung and lymphoid tissues, but no or low expression in antigen-presenting cells including Langerhans' cells of the skin. The high abundance of alpha 2MR/LRP in certain cell types of most organs suggests two main routes for alpha 2MR/LRP-mediated ligand clearance: (1) systemic removal in liver of circulating ligands, and (2) non-hepatic interstitial removal in different organs, including the brain.

MeSH Terms
Antibodies, Monoclonal Astrocytes/chemistry,ultrastructure Digestive System/chemistry,ultrastructure Female Humans Immunohistochemistry Kidney/chemistry,ultrastructure Langerhans Cells/chemistry,ultrastructure Low Density Lipoprotein Receptor-Related Protein-1 Male Muscles/chemistry,ultrastructure Neurons/chemistry,ultrastructure Ovary/chemistry,ultrastructure Phagocytes/chemistry,ultrastructure Receptors, Immunologic/analysis Testis/chemistry,ultrastructure
Chemicals
Antibodies, Monoclonal Low Density Lipoprotein Receptor-Related Protein-1 Receptors, Immunologic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Moestrup S K
Institute of Medical Biochemistry, Aarhus University, Denmark.
Gliemann J
Pallesen G
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00353892
Published
1992-09-00
Pages
375-82
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00353892 View journal details
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