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PMID: 8178930 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of cloned aortic smooth muscle cells from young rats.

The American journal of pathology ·Vol. 144 ·No. 5 ·1994-05-00 ·Pages 1068-81

Lemire JM, Covin CW, White S, Giachelli CM, Schwartz SM

Abstract

Clones were derived by dilute plating from cultured aortic smooth muscle cells of 12-day-old rats. Clones Pup I to III resemble uncloned smooth muscle cultures from aortas of rat pups and from adult rat neointimas. They have a cobblestone morphology and proliferate in plasma-derived serum. By Northern analysis they contain platelet-derived growth factor B chain (PDGF-B) mRNA and high levels of CYPIA1, elastin, and osteopontin mRNAs, and they lack platelet-derived growth factor alpha-receptor (PDGF-alpha R) mRNA. In contrast, Pup V resembles smooth muscle cultures derived from uninjured adult rat arteries. It has an elongated morphology and proliferates poorly in plasma-derived serum. This clone expresses PDGF-alpha R mRNA, low levels of elastin and osteopontin mRNAs, and lacks CYPIA1 and PDGF-B mRNAs. Pup IV and VI have most of the properties of Pup I to III. We conclude that uncloned pup cultures are heterogeneous, but that the growth properties and gene expression pattern described for the uncloned culture is characteristic of individual clones within the population.

MeSH Terms
Actins/analysis Animals Aorta/chemistry,cytology,injuries Base Sequence Cell Division/genetics Clone Cells Cytochrome P-450 CYP1A1 Cytochrome P-450 Enzyme System/genetics Elastin/genetics Gene Expression Regulation Male Molecular Sequence Data Muscle, Smooth, Vascular/chemistry,cytology,injuries Myosins/analysis Osteopontin Oxidoreductases/genetics Phenotype Platelet-Derived Growth Factor/genetics Rats Rats, Sprague-Dawley Rats, Wistar Sialoglycoproteins/genetics
Chemicals
Actins Platelet-Derived Growth Factor Sialoglycoproteins Spp1 protein, rat Osteopontin Elastin Cytochrome P-450 Enzyme System Oxidoreductases Cytochrome P-450 CYP1A1 Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lemire J M
Department of Pathology, University of Washington School of Medicine, Seattle 98195.
Covin C W
White S
Giachelli C M
Schwartz S M
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1994-05-00
Pages
1068-81
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1887366
Subset
IM
Grants
NHLBI NIH HHS · 2 PO1 HL-03174 · United States
NHLBI NIH HHS · 2 PO1 HL-18645 · United States
NHLBI NIH HHS · 2T32 HL-07312 · United States
External Links
PubMed source PMC Full Text View journal details
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