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PMID: 8170978 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Molecular cloning and tissue distribution of PHAS-I, an intracellular target for insulin and growth factors.

Hu C, Pang S, Kong X, Velleca M, Lawrence JC

Abstract

Although the actions of insulin and a number of growth factors that signal via protein-tyrosine kinase receptors are believed to involve increased phosphorylation of key intracellular proteins, relatively few of the downstream phosphoproteins have been identified. In this report we describe a cDNA encoding one of the most prominent insulin-stimulated phosphoproteins in rat adipocytes. The cDNA encodes a protein, designated PHAS-I, which has 117 amino acids and a M(r) of 12,400. When translated in vitro and subjected to SDS/PAGE, PHAS-I migrates anomalously, having an apparent M(r) of 21,000. The predicted amino acid composition is interesting in that approximately 45% of the PHAS-I protein is accounted for by only four amino acids--serine, threonine, proline, and glycine. The PHAS-I gene is expressed in a variety of tissues, although the highest levels of mRNA are present in fat and skeletal muscle, two of the most insulin-responsive tissues. The nucleotide and deduced amino acid sequences of PHAS-I differ from any that have been reported, and homology screening provided no clues concerning the function of the protein. However, in view of its tissue distribution and the fact that the protein is phosphorylated in response to insulin, we speculate that PHAS-I is important in insulin action.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Carrier Proteins Cloning, Molecular DNA Primers/chemistry DNA, Complementary/genetics Gene Expression Genes Growth Substances/pharmacology Insulin/pharmacology Intracellular Signaling Peptides and Proteins Male Molecular Sequence Data Phosphoproteins/genetics,metabolism RNA, Messenger/genetics Rats Rats, Sprague-Dawley Tissue Distribution
Chemicals
Carrier Proteins DNA Primers DNA, Complementary Eif4ebp1 protein, rat Growth Substances Insulin Intracellular Signaling Peptides and Proteins Phosphoproteins RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hu C
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Pang S
Kong X
Velleca M
Lawrence J C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1994-04-26
Pages
3730-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43655
Subset
IM
Grants
NIDDK NIH HHS · DK28312 · United States
Databases
GENBANK
U05014
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