Abstract
Although the actions of insulin and a number of growth factors that signal via protein-tyrosine kinase receptors are believed to involve increased phosphorylation of key intracellular proteins, relatively few of the downstream phosphoproteins have been identified. In this report we describe a cDNA encoding one of the most prominent insulin-stimulated phosphoproteins in rat adipocytes. The cDNA encodes a protein, designated PHAS-I, which has 117 amino acids and a M(r) of 12,400. When translated in vitro and subjected to SDS/PAGE, PHAS-I migrates anomalously, having an apparent M(r) of 21,000. The predicted amino acid composition is interesting in that approximately 45% of the PHAS-I protein is accounted for by only four amino acids--serine, threonine, proline, and glycine. The PHAS-I gene is expressed in a variety of tissues, although the highest levels of mRNA are present in fat and skeletal muscle, two of the most insulin-responsive tissues. The nucleotide and deduced amino acid sequences of PHAS-I differ from any that have been reported, and homology screening provided no clues concerning the function of the protein. However, in view of its tissue distribution and the fact that the protein is phosphorylated in response to insulin, we speculate that PHAS-I is important in insulin action.
MeSH Terms
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins
Cloning, Molecular
DNA Primers/chemistry
DNA, Complementary/genetics
Gene Expression
Genes
Growth Substances/pharmacology
Insulin/pharmacology
Intracellular Signaling Peptides and Proteins
Male
Molecular Sequence Data
Phosphoproteins/genetics,metabolism
RNA, Messenger/genetics
Rats
Rats, Sprague-Dawley
Tissue Distribution
Chemicals
Carrier Proteins
DNA Primers
DNA, Complementary
Eif4ebp1 protein, rat
Growth Substances
Insulin
Intracellular Signaling Peptides and Proteins
Phosphoproteins
RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hu C
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110.
Pang S
Kong X
Velleca M
Lawrence J C
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