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PMID: 8169206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mutations in pts cause catabolite-resistant sporulation and altered regulation of spo0H in Bacillus subtilis.

Journal of bacteriology ·Vol. 176 ·No. 9 ·1994-05-00 ·Pages 2587-95

Frisby D, Zuber P

Abstract

A mutation in Bacillus subtilis, ggr-31, that relieves glucose-glutamine-dependent control of a spoVG-lacZ translational fusion was isolated and was subsequently found to confer a pleiotropic phenotype. Mutants cultured in glucose- and glutamine-rich media exhibited a Crs- (catabolite-resistant sporulation) phenotype; enhanced expression of the spo0H gene, encoding sigma H, as evidenced by immunoblot analysis with anti-sigma H antiserum; and derepression of srfA, an operon involved in surfactin biosynthesis and competence development. In addition, ggr-31 mutants exhibited a significant increase in generation time when they were cultured in minimal glucose medium. The mutant phenotype was restored to the wild type by Campbell integration of a plasmid containing part of the ptsG (encoding the enzyme II/III glucose permease) gene, indicating that the mutation probably resides within ptsG and adversely affects glucose uptake. A deletion mutation within ptsI exhibited a phenotype similar to that of ggr-31.

Related Genes
ptsG spo0G
MeSH Terms
Bacillus subtilis/genetics,growth & development Bacterial Proteins/biosynthesis,genetics Base Sequence Gene Expression Regulation, Bacterial/drug effects Glucose/pharmacology Glutamine/pharmacology Molecular Sequence Data Phenotype Phosphoenolpyruvate Sugar Phosphotransferase System/genetics RNA, Messenger/analysis Recombinant Fusion Proteins/biosynthesis Restriction Mapping Sigma Factor/analysis Spores, Bacterial/genetics,growth & development Transcription Factors Transcription, Genetic
Chemicals
Bacterial Proteins RNA, Messenger Recombinant Fusion Proteins Sigma Factor Transcription Factors spoIIR protein, Bacillus subtilis spore-specific proteins, Bacillus Glutamine Phosphoenolpyruvate Sugar Phosphotransferase System Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Frisby D
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.
Zuber P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.9.2587-2595.1994
Published
1994-05-00
Pages
2587-95
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205396
Subset
IM
Grants
NIGMS NIH HHS · GM39479 · United States
NIGMS NIH HHS · GM45898 · United States
External Links
PubMed source DOI: 10.1128/jb.176.9.2587-2595.1994 PMC Full Text View journal details
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