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PMID: 1577686 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity.

Journal of bacteriology ·Vol. 174 ·No. 10 ·1992-05-00 ·Pages 3161-70

Arnaud M, Vary P, Zagorec M, Klier A, Debarbouille M, Postma P, Rapoport G

Abstract

The sacT gene which controls the sacPA operon of Bacillus subtilis encodes a polypeptide homologous to the B. subtilis SacY and the Escherichia coli BglG antiterminators. Expression of the sacT gene is shown to be constitutive. The DNA sequence upstream from sacP contains a palindromic sequence which functions as a transcriptional terminator. We have previously proposed that SacT acts as a transcriptional antiterminator, allowing transcription of the sacPA operon. In strains containing mutations inactivating ptsH or ptsI, the expression of sacPA and sacB is constitutive. In this work, we show that this constitutivity is due to a fully active SacY antiterminator. In the wild-type sacT+ strain or in the sacT30 mutant, SacT requires both enzyme I and HPr of the phosphotransferase system (PTS) for antitermination. It appears that the PTS exerts different effects on the sacB gene and the sacPA operon. The general proteins of the PTS are not required for the activity of SacY while they are necessary for SacT activity.

Related Genes
SacT
MeSH Terms
Bacillus subtilis/genetics Bacterial Proteins/genetics Base Sequence DNA Mutational Analysis Gene Expression Regulation, Bacterial/genetics Genes, Regulator/genetics Lac Operon/genetics Models, Genetic Molecular Sequence Data Mutagenesis, Site-Directed Operon/genetics Phosphoenolpyruvate Sugar Phosphotransferase System/genetics RNA-Binding Proteins/genetics Recombinant Proteins/analysis Restriction Mapping Terminator Regions, Genetic beta-Galactosidase/analysis
Chemicals
Bacterial Proteins RNA-Binding Proteins Recombinant Proteins antiterminator proteins, Bacteria Phosphoenolpyruvate Sugar Phosphotransferase System beta-Galactosidase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Arnaud M
Laboratoire de Biochimie Microbienne, Institute Pasteur, URA 1300, Centre National de la Recherche Scientifique, Paris, France.
Vary P
Zagorec M
Klier A
Debarbouille M
Postma P
Rapoport G
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.10.3161-3170.1992
Published
1992-05-00
Pages
3161-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205982
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.10.3161-3170.1992 PMC Full Text View journal details
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