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PMID: 1557038 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of sfp: a gene that functions in the production of the lipopeptide biosurfactant, surfactin, in Bacillus subtilis.

Molecular & general genetics : MGG ·Vol. 232 ·No. 2 ·1992-03-00 ·Pages 313-21

Nakano MM, Corbell N, Besson J, Zuber P

Abstract

The sfp gene is required for cells of Bacillus subtilis to become producers of the lipopeptide antibiotic surfactin. sfp was isolated and its nucleotide sequence was determined. sfp was expressed in Escherichia coli and its putative product was purified for use in antibody production and in amino acid sequence analysis. The gene was plasmid-amplified in B. subtilis, where it conferred a Srf+ phenotype on sfp0 (surfactin non-producing) cells. Overproduction of Sfp in B. subtilis did not cause production of an increased amount of surfactin and resulted in the repression of a lacZ transcriptional fusion of the srfA operon, which encodes enzymes that catalyze surfactin synthesis. We propose that sfp represents an essential component of peptide synthesis systems and also plays a role, either directly or indirectly, in the regulation of surfactin biosynthesis gene expression.

Related Genes
lacZ sfp
MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics,metabolism Bacterial Proteins/biosynthesis Base Sequence DNA, Bacterial/genetics Electrophoresis, Polyacrylamide Gel Gene Expression Genes, Bacterial Lipopeptides Molecular Sequence Data Operon Peptides, Cyclic Plasmids Restriction Mapping Transcription, Genetic Transduction, Genetic Transformation, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial Lipopeptides Peptides, Cyclic surfactin peptide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nakano M M
Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport 71130-3932.
Corbell N
Besson J
Zuber P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00280011
Published
1992-03-00
Pages
313-21
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM39476 · United States
Databases
GENBANK
X63158
External Links
PubMed source DOI: 10.1007/BF00280011
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