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PMID: 2477357 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gramicidin S biosynthesis operon containing the structural genes grsA and grsB has an open reading frame encoding a protein homologous to fatty acid thioesterases.

Journal of bacteriology ·Vol. 171 ·No. 10 ·1989-10-00 ·Pages 5422-9

Krätzschmar J, Krause M, Marahiel MA

Abstract

The DNA sequence of about 5.9 kilobase pairs (kbp) of the gramicidin S biosynthesis operon (grs) was determined. Three open reading frames were identified; the corresponding genes, called grsT, grsA, and grsB, were found to be organized in one transcriptional unit, not two as previously reported (M. Krause and M. A. Marahiel, J. Bacteriol. 170:4669-4674, 1988). The entire nucleotide sequence of grsA, coding for the 126.663-kilodalton gramicidin S synthetase 1, grsT, encoding a 29.191-kilodalton protein of unknown function, and 732 bp of the 5' end of grsB, encoding the gramicidin S synthetase 2, were determined. A single initiation site of transcription 81 bp upstream of the grsT initiation condon GTG was identified by high-resolution S1 mapping studies. The sequence of the grsA gene product showed a high degree of homology to the tyrocidine synthetase 1 (TycA protein), and that of grsT exhibited a significant degree of homology to vertebrate fatty acid thioesterases.

MeSH Terms
Amino Acid Sequence Bacillus/genetics Bacterial Proteins/genetics Base Sequence Genes, Bacterial Gramicidin/metabolism Molecular Sequence Data Molecular Weight Operon Palmitoyl-CoA Hydrolase/genetics Promoter Regions, Genetic Restriction Mapping Single-Strand Specific DNA and RNA Endonucleases Thiolester Hydrolases Transcription, Genetic
Chemicals
Bacterial Proteins grsT protein, Bacillus brevis Gramicidin Thiolester Hydrolases Palmitoyl-CoA Hydrolase Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Krätzschmar J
Institut für Biochemie und Molekulare Biologie, Technische Universität Berlin, Federal Republic of Germany.
Krause M
Marahiel M A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.10.5422-5429.1989
Published
1989-10-00
Pages
5422-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210379
Subset
IM
Databases
GENBANK
M29703, X15577
External Links
PubMed source DOI: 10.1128/jb.171.10.5422-5429.1989 PMC Full Text View journal details
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