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PMID: 815269 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae.

Journal of clinical microbiology ·Vol. 3 ·No. 1 ·1976-01-00 ·Pages 8-13

Morse SA, Bartenstein L

Abstract

A suspending medium was developed for use with the Minitek system for the confirmatory identification of Neisseria gonorrhoeae, N. meningitidis, and N. lactamica based upon the production of acid from various carbohydrates. The addition of sodium bicarbonate to the medium made negative reactions easier to read. More isolates of N. gonorrhoeae were identified with the suspending medium in the Minitek system than with cystine-Trypticase agar media. With a suitable inoculum size, a positive identification could be made in less than 1 h; most isolates (90,8%) could be identified within 4 h of inoculation. The Minitek system is reliable and easy to use.

MeSH Terms
Acids/biosynthesis Bacteriological Techniques Evaluation Studies as Topic Glucose/metabolism Maltose/metabolism Neisseria/isolation & purification,metabolism Neisseria gonorrhoeae/isolation & purification,metabolism Neisseria meningitidis/isolation & purification,metabolism Nitrates/pharmacology Species Specificity Sucrose/metabolism
Chemicals
Acids Nitrates Sucrose Maltose Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morse S A
Bartenstein L
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23 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.3.1.8-13.1976
Published
1976-01-00
Pages
8-13
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC274218
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.3.1.8-13.1976 PMC Full Text View journal details
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