Home LiteratureArticle Details
PMID: 8138575 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sar1 promotes vesicle budding from the endoplasmic reticulum but not Golgi compartments.

The Journal of cell biology ·Vol. 125 ·No. 1 ·1994-04-00 ·Pages 51-65

Kuge O, Dascher C, Orci L, Rowe T, Amherdt M, Plutner H, Ravazzola M, Tanigawa G, Rothman JE, Balch WE

Abstract

Two new members (Sar1a and Sar1b) of the SAR1 gene family have been identified in mammalian cells. Using immunoelectron microscopy, Sar1 was found to be restricted to the transitional region where the protein was enriched 20-40-fold in vesicular carriers mediating ER to Golgi traffic. Biochemical analysis revealed that Sar1 was essential for an early step in vesicle budding. A Sar1-specific antibody potently inhibited export of vesicular stomatitis virus glycoprotein (VSV-G) from the ER in vitro. Consistent with the role of guanine nucleotide exchange in Sar1 function, a trans-dominant mutant (Sar1a[T39N]) with a preferential affinity for GDP also strongly inhibited vesicle budding from the ER. In contrast, Sar1 was not found to be required for the transport of VSV-G between sequential Golgi compartments, suggesting that components active in formation of vesicular carriers mediating ER to Golgi traffic may differ, at least in part, from those involved in intra-Golgi transport. The requirement for novel components at different stages of the secretory pathway may reflect the recently recognized differences in protein transport between the Golgi stacks as opposed to the selective sorting and concentration of protein during export from the ER.

Related Genes
ARF1 H-ras SAR1 Sar1a Sar1b rab1 rab2 rab6
MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Transport CHO Cells Cloning, Molecular Consensus Sequence Cricetinae DNA Primers/chemistry Endoplasmic Reticulum/metabolism GTP Phosphohydrolases/metabolism GTP-Binding Proteins/metabolism Golgi Apparatus/metabolism Guanosine Triphosphate/metabolism HeLa Cells Humans Intracellular Membranes/metabolism Islets of Langerhans/metabolism Membrane Glycoproteins/metabolism Molecular Sequence Data Monomeric GTP-Binding Proteins Multigene Family Rats Saccharomyces cerevisiae Proteins Sequence Alignment Sequence Homology, Amino Acid Vesicular Transport Proteins
Chemicals
DNA Primers Membrane Glycoproteins Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Guanosine Triphosphate GTP Phosphohydrolases GTP-Binding Proteins SAR1 protein, rat Monomeric GTP-Binding Proteins SAR1 protein, S cerevisiae
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Kuge O
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021.
Dascher C
Orci L
Rowe T
Amherdt M
Plutner H
Ravazzola M
Tanigawa G
Rothman J E
Balch W E
References (80)
80 references, click to expand
  1. Dissection of a single round of vesicular transport: sequential intermediates for intercisternal movement in the Golgi stack.
    Cell. 1989 Feb 10;56(3):357-68 PMID: 2536591
  2. Mammalian Sec23p homologue is restricted to the endoplasmic reticulum transitional cytoplasm.
    Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8611-5 PMID: 1924322
  3. Semi-intact cells permeable to macromolecules: use in reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex.
    Cell. 1987 Aug 14;50(4):523-34 PMID: 3038335
  4. Reconstitution of the transport of protein between successive compartments of the Golgi measured by the coupled incorporation of N-acetylglucosamine.
    Cell. 1984 Dec;39(2 Pt 1):405-16 PMID: 6498939
  5. Inhibition by brefeldin A of a Golgi membrane enzyme that catalyses exchange of guanine nucleotide bound to ARF.
    Nature. 1992 Nov 26;360(6402):352-4 PMID: 1448152
  6. The ras protein family: evolutionary tree and role of conserved amino acids.
    Biochemistry. 1991 May 14;30(19):4637-48 PMID: 2029511
  7. Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II.
    J Cell Biol. 1993 Jul;122(1):39-51 PMID: 8314846
  8. Binding of coatomer to Golgi membranes requires ADP-ribosylation factor.
    J Biol Chem. 1993 Jun 5;268(16):12083-9 PMID: 8505331
  9. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of a yeast coatomer.
    Nature. 1992 Dec 10;360(6404):603-5 PMID: 1461285
  10. SNAP receptors implicated in vesicle targeting and fusion.
    Nature. 1993 Mar 25;362(6418):318-24 PMID: 8455717
  11. Biological and biochemical properties of human rasH genes mutated at codon 61.
    Cell. 1986 Jan 17;44(1):167-76 PMID: 3510078
  12. Reconstitution of SEC gene product-dependent intercompartmental protein transport.
    Cell. 1988 Jul 29;54(3):335-44 PMID: 3293799
  13. Identification of an intermediate compartment involved in protein transport from endoplasmic reticulum to Golgi apparatus.
    Eur J Cell Biol. 1990 Dec;53(2):185-96 PMID: 1964413
  14. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway.
    Cell. 1990 May 18;61(4):723-33 PMID: 2188733
  15. Application of cryoultramicrotomy to immunocytochemistry.
    J Microsc. 1986 Aug;143(Pt 2):139-49 PMID: 3531524
  16. A membrane glycoprotein, Sec12p, required for protein transport from the endoplasmic reticulum to the Golgi apparatus in yeast.
    J Cell Biol. 1988 Sep;107(3):851-63 PMID: 3047151
  17. Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus.
    J Biol Chem. 1994 Jan 14;269(2):1437-48 PMID: 8288610
  18. Requirements for cholera toxin-dependent ADP-ribosylation of the purified regulatory component of adenylate cyclase.
    J Biol Chem. 1982 Jan 10;257(1):20-3 PMID: 6273425
  19. Molecular dissection of the secretory pathway.
    Nature. 1992 Jan 30;355(6359):409-15 PMID: 1734280
  20. Rab1b regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.
    J Cell Biol. 1991 Oct;115(1):31-43 PMID: 1918138
  21. Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.
    EMBO J. 1990 Aug;9(8):2351-9 PMID: 2196171
  22. Mutational analysis of SEC4 suggests a cyclical mechanism for the regulation of vesicular traffic.
    EMBO J. 1989 Jun;8(6):1685-93 PMID: 2504585
  23. A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast.
    Nature. 1989 Jun 1;339(6223):355-9 PMID: 2657434
  24. Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.
    J Histochem Cytochem. 1978 Dec;26(12):1074-81 PMID: 366014
  25. Beta-COP is essential for biosynthetic membrane transport from the endoplasmic reticulum to the Golgi complex in vivo.
    Cell. 1993 Jul 16;74(1):71-82 PMID: 8334707
  26. Immunochemistry on ultrathin frozen sections.
    Histochem J. 1980 Jul;12(4):381-403 PMID: 7440248
  27. A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2677-91 PMID: 2512296
  28. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery.
    Cell. 1988 Mar 25;52(6):915-24 PMID: 3127057
  29. Distribution of the intermediate elements operating in ER to Golgi transport.
    J Cell Sci. 1991 Nov;100 ( Pt 3):415-30 PMID: 1808196
  30. ADP-ribosylation factor, a small GTP-binding protein, is required for binding of the coatomer protein beta-COP to Golgi membranes.
    Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6408-12 PMID: 1631136
  31. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein.
    Nature. 1992 Nov 26;360(6402):350-2 PMID: 1448151
  32. ADP-ribosylation factor is functionally and physically associated with the Golgi complex.
    Proc Natl Acad Sci U S A. 1990 Feb;87(3):1238-42 PMID: 2105501
  33. SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER.
    Nature. 1993 Sep 23;365(6444):347-9 PMID: 8377826
  34. The GTP-binding Sar1 protein is localized to the early compartment of the yeast secretory pathway.
    Biochim Biophys Acta. 1991 Jul 10;1093(2-3):135-43 PMID: 1907491
  35. Differential inhibition of multiple vesicular transport steps between the endoplasmic reticulum and trans Golgi network.
    J Biol Chem. 1993 Feb 25;268(6):4216-26 PMID: 8382697
  36. Purification and characterization of SAR1p, a small GTP-binding protein required for transport vesicle formation from the endoplasmic reticulum.
    J Biol Chem. 1993 Jan 15;268(2):873-9 PMID: 8419365
  37. Preferential inhibition of the oncogenic form of RasH by mutations in the GAP binding/"effector" domain.
    Cell. 1991 Feb 8;64(3):625-33 PMID: 1899358
  38. Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calcium.
    Cell. 1988 May 20;53(4):635-47 PMID: 3286011
  39. The oncogenic activation of human p21ras by a novel mechanism.
    Science. 1986 Aug 8;233(4764):649-52 PMID: 3487832
  40. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
    Proc Natl Acad Sci U S A. 1986 Nov;83(21):8122-6 PMID: 3095828
  41. The isolated ER-Golgi intermediate compartment exhibits properties that are different from ER and cis-Golgi.
    J Cell Biol. 1991 Apr;113(1):45-54 PMID: 2007626
  42. ATP-coupled transport of vesicular stomatitis virus G protein between the endoplasmic reticulum and the Golgi.
    J Biol Chem. 1986 Nov 5;261(31):14681-9 PMID: 3021750
  43. The GTPase superfamily: conserved structure and molecular mechanism.
    Nature. 1991 Jan 10;349(6305):117-27 PMID: 1898771
  44. ras genes.
    Annu Rev Biochem. 1987;56:779-827 PMID: 3304147
  45. Requirement for a GTPase-activating protein in vesicle budding from the endoplasmic reticulum.
    Science. 1993 Mar 5;259(5100):1466-8 PMID: 8451644
  46. Role of an N-ethylmaleimide-sensitive transport component in promoting fusion of transport vesicles with cisternae of the Golgi stack.
    Cell. 1988 Jul 15;54(2):221-7 PMID: 3390865
  47. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  48. Small GTP-binding proteins in vesicular transport.
    Trends Biochem Sci. 1990 Dec;15(12):473-7 PMID: 2077687
  49. Morphological analysis of protein transport from the ER to Golgi membranes in digitonin-permeabilized cells: role of the P58 containing compartment.
    J Cell Biol. 1992 Dec;119(5):1097-116 PMID: 1447290
  50. DSS4-1 is a dominant suppressor of sec4-8 that encodes a nucleotide exchange protein that aids Sec4p function.
    Nature. 1993 Feb 4;361(6411):460-3 PMID: 8429886
  51. GTP-binding mutants of rab1 and rab2 are potent inhibitors of vesicular transport from the endoplasmic reticulum to the Golgi complex.
    J Cell Biol. 1992 Nov;119(4):749-61 PMID: 1429835
  52. Budding from Golgi membranes requires the coatomer complex of non-clathrin coat proteins.
    Nature. 1993 Apr 15;362(6421):648-52 PMID: 8464517
  53. Inhibition of NIH 3T3 cell proliferation by a mutant ras protein with preferential affinity for GDP.
    Mol Cell Biol. 1988 Aug;8(8):3235-43 PMID: 3145408
  54. Identification, by a monoclonal antibody, of a 53-kD protein associated with a tubulo-vesicular compartment at the cis-side of the Golgi apparatus.
    J Cell Biol. 1988 Nov;107(5):1643-53 PMID: 3182932
  55. Trimeric G proteins and vesicle formation.
    Trends Cell Biol. 1992 Apr;2(4):91-4 PMID: 14732001
  56. Sequential transport of protein between the endoplasmic reticulum and successive Golgi compartments in semi-intact cells.
    J Biol Chem. 1991 Jul 15;266(20):13055-63 PMID: 1649174
  57. A coat subunit of Golgi-derived non-clathrin-coated vesicles with homology to the clathrin-coated vesicle coat protein beta-adaptin.
    Nature. 1991 Jan 17;349(6306):215-20 PMID: 1898984
  58. Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.
    J Biol Chem. 1993 Jan 15;268(2):923-9 PMID: 8419371
  59. The binding of AP-1 clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small GTP-binding protein.
    Cell. 1993 Jun 4;73(5):999-1005 PMID: 8500185
  60. The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins.
    EMBO J. 1993 Nov;12(11):4073-82 PMID: 8223424
  61. Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro.
    J Cell Biol. 1993 Feb;120(4):865-75 PMID: 8432727
  62. 'Coatomer': a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles.
    Nature. 1991 Jan 17;349(6306):248-51 PMID: 1898986
  63. Sec12p-dependent membrane binding of the small GTP-binding protein Sar1p promotes formation of transport vesicles from the ER.
    J Cell Biol. 1991 Aug;114(4):663-70 PMID: 1907973
  64. A general method of in vitro preparation and specific mutagenesis of DNA fragments: study of protein and DNA interactions.
    Nucleic Acids Res. 1988 Aug 11;16(15):7351-67 PMID: 3045756
  65. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway.
    Cell. 1980 Aug;21(1):205-15 PMID: 6996832
  66. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation.
    Nature. 1989 Sep 21;341(6239):209-14 PMID: 2476675
  67. Brefeldin A, a drug that blocks secretion, prevents the assembly of non-clathrin-coated buds on Golgi cisternae.
    Cell. 1991 Mar 22;64(6):1183-95 PMID: 2004424
  68. Beta-COP is essential for transport of protein from the endoplasmic reticulum to the Golgi in vitro.
    J Cell Biol. 1993 Sep;122(6):1155-67 PMID: 8376457
  69. Yeast Sec23p acts in the cytoplasm to promote protein transport from the endoplasmic reticulum to the Golgi complex in vivo and in vitro.
    EMBO J. 1989 Jun;8(6):1677-84 PMID: 2670558
  70. Reconstitution of GTP-binding Sar1 protein function in ER to Golgi transport.
    J Cell Biol. 1991 Aug;114(4):671-9 PMID: 1907974
  71. Fission yeast and a plant have functional homologues of the Sar1 and Sec12 proteins involved in ER to Golgi traffic in budding yeast.
    EMBO J. 1992 Nov;11(11):4205-11 PMID: 1396601
  72. The amino terminus of ADP-ribosylation factor (ARF) is a critical determinant of ARF activities and is a potent and specific inhibitor of protein transport.
    J Biol Chem. 1992 Jun 25;267(18):13039-46 PMID: 1618801
  73. A soluble secretory protein is first concentrated in the endoplasmic reticulum before transfer to the Golgi apparatus.
    Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5732-6 PMID: 8390678
  74. Rab GTPases in vesicular transport.
    Curr Opin Cell Biol. 1993 Aug;5(4):613-20 PMID: 8257602
  75. Envelope proteins of vesicular stomatitis virus: effect of temperature-sensitive mutations in complementation groups III and V.
    J Virol. 1974 Nov;14(5):1220-8 PMID: 4372395
  76. Role of heterotrimeric G proteins in membrane traffic.
    Mol Biol Cell. 1992 Dec;3(12):1317-28 PMID: 1493332
  77. Compartmental organization of Golgi-specific protein modification and vacuolar protein sorting events defined in a yeast sec18 (NSF) mutant.
    J Cell Biol. 1991 Jul;114(2):207-18 PMID: 2071670
  78. The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization.
    Cell. 1986 Nov 7;47(3):401-12 PMID: 3094963
  79. Two distinct members of the ADP-ribosylation factor family of GTP-binding proteins regulate cell-free intra-Golgi transport.
    Cell. 1992 Jul 10;70(1):69-79 PMID: 1623523
  80. Sec23p and a novel 105-kDa protein function as a multimeric complex to promote vesicle budding and protein transport from the endoplasmic reticulum.
    Mol Biol Cell. 1992 Jun;3(6):667-76 PMID: 1498369
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.125.1.51
Published
1994-04-00
Pages
51-65
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120015
Subset
IM
Grants
NCI NIH HHS · CA47767 · United States
NIGMS NIH HHS · GM 42336 · United States
NCRR NIH HHS · RR07273 · United States
External Links
PubMed source DOI: 10.1083/jcb.125.1.51 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2