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PMID: 8314846 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II.

The Journal of cell biology ·Vol. 122 ·No. 1 ·1993-07-00 ·Pages 39-51

Velasco A, Hendricks L, Moremen KW, Tulsiani DR, Touster O, Farquhar MG

Abstract

alpha-mannosidases I and II (Man I and II) are resident enzymes of the Golgi complex involved in oligosaccharide processing during N-linked glycoprotein biosynthesis that are widely considered to be markers of the cis- and medial-Golgi compartments, respectively. We have investigated the distribution of these enzymes in several cell types by immunofluorescence and immunoelectron microscopy. Man II was most commonly found in medial- and/or trans- cisternae but showed cell type-dependent variations in intra-Golgi distribution. It was variously localized to either medial (NRK and CHO cells), both medial and trans (pancreatic acinar cells, enterocytes), or trans- (goblet cells) cisternae, or distributed across the entire Golgi stack (hepatocytes and some enterocytes). The distribution of Man I largely coincided with that of Man II in that it was detected primarily in medial- and trans-cisternae. It also showed cell type dependent variations in its intra-Golgi distribution. Man I and Man II were also detected within secretory granules and at the cell surface of some cell types (enterocytes, pancreatic acinar cells, goblet cells). In the case of Man II, cell surface staining was shown not to be due to antibody cross-reactivity with oligosaccharide epitopes. These results indicate that the distribution of Man I and Man II within the Golgi stack of a given cell type overlaps considerably, and their distribution from one cell type to another is more variable and less compartmentalized than previously assumed.

MeSH Terms
Animals Cell Line Cell Membrane/enzymology,ultrastructure Epithelium/enzymology,ultrastructure Fluorescent Antibody Technique Golgi Apparatus/enzymology,ultrastructure Immunoenzyme Techniques Immunohistochemistry Intestinal Mucosa/enzymology,ultrastructure Kidney Liver/enzymology,ultrastructure Mannosidases/analysis,metabolism Microscopy, Immunoelectron Multiple Myeloma Organ Specificity Pancreas/enzymology,ultrastructure Rats Subcellular Fractions/enzymology,ultrastructure Tumor Cells, Cultured
Chemicals
Mannosidases mannosyl-oligosaccharide 1,2-alpha-mannosidase mannosyl-oligosaccharide 1,3 - 1,6-alpha-mannosidase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Velasco A
Division of Cellular and Molecular Medicine and Center for Molecular Genetics, University of California, San Diego, La Jolla 92093-0651.
Hendricks L
Moremen K W
Tulsiani D R
Touster O
Farquhar M G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.122.1.39
Published
1993-07-00
Pages
39-51
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119607
Subset
IM
Grants
NCI NIH HHS · CA46128 · United States
NIDDK NIH HHS · DK17780 · United States
NIGMS NIH HHS · GM26430 · United States
External Links
PubMed source DOI: 10.1083/jcb.122.1.39 PMC Full Text View journal details
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