Home LiteratureArticle Details
PMID: 8114726 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription activates RecA-promoted homologous pairing of nucleosomal DNA.

Molecular and cellular biology ·Vol. 14 ·No. 3 ·1994-03-00 ·Pages 1949-55

Kotani H, Kmiec EB

Abstract

RecA protein catalyzes the homologous pairing of a single-stranded circular DNA and a linear duplex DNA molecule. When the duplex is packaged into chromatin, formation of homologously paired complexes is blocked. We have established a system for studying the RecA-promoted reaction by using a duplex fragment containing a single-phased nucleosome. Under these conditions there is no reaction leading to formation of joint molecule complexes. However, transcription on the chromatin template activates the formation of complexes. Reaction is dependent on RNA synthesis and DNA sequence homology and proceeds regardless of the direction of transcription.

MeSH Terms
Cell-Free System Chromatin/ultrastructure DNA-Directed RNA Polymerases/metabolism In Vitro Techniques Nucleosomes/physiology Rec A Recombinases/metabolism Recombination, Genetic Templates, Genetic Transcription, Genetic
Chemicals
Chromatin Nucleosomes Rec A Recombinases DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kotani H
Department of Pharmacology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
Kmiec E B
References (28)
28 references, click to expand
  1. Kinetic analysis of deoxyribonuclease I cleavages in the nucleosome core: evidence for a DNA superhelix.
    J Mol Biol. 1978 Sep 15;124(2):391-420 PMID: 568667
  2. Transcription enhances intrachromosomal homologous recombination in mammalian cells.
    Mol Cell Biol. 1992 Dec;12(12):5311-8 PMID: 1333040
  3. A simple procedure for parallel sequence analysis of both strands of 5'-labeled DNA.
    Gene. 1983 Aug;23(2):175-83 PMID: 6311685
  4. The role of stable complexes that repress and activate eucaryotic genes.
    Cell. 1984 Jun;37(2):359-65 PMID: 6722879
  5. A bacteriophage RNA polymerase transcribes through a Xenopus 5S RNA gene transcription complex without disrupting it.
    Cell. 1986 Feb 14;44(3):381-9 PMID: 3943130
  6. Structural analysis of a triple complex between the histone octamer, a Xenopus gene for 5S RNA and transcription factor IIIA.
    EMBO J. 1985 Dec 16;4(13A):3473-82 PMID: 4092686
  7. DNA bending and its relation to nucleosome positioning.
    J Mol Biol. 1985 Dec 20;186(4):773-90 PMID: 3912515
  8. Transcriptional activities of mammalian genomes at sites of recombination with foreign DNA.
    J Virol. 1987 Feb;61(2):344-53 PMID: 3806790
  9. Recombination-stimulating sequences in yeast ribosomal DNA correspond to sequences regulating transcription by RNA polymerase I.
    Cell. 1987 Mar 27;48(6):1071-9 PMID: 3548996
  10. Nucleosomes inhibit the initiation of transcription but allow chain elongation with the displacement of histones.
    Cell. 1987 Apr 24;49(2):203-10 PMID: 3568125
  11. A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it.
    Cell. 1987 Aug 28;50(5):801-8 PMID: 3621345
  12. Development of the primary antibody repertoire.
    Science. 1987 Nov 20;238(4830):1079-87 PMID: 3317825
  13. Sensitive homologous recombination strand-transfer assay: partial purification of a Drosophila melanogaster enzyme and detection of sequence effects on the strand-transfer activity of RecA protein.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):5854-8 PMID: 3137561
  14. Elevated recombination rates in transcriptionally active DNA.
    Cell. 1989 Feb 24;56(4):619-30 PMID: 2645056
  15. DNase I sensitivity of immunoglobulin light chain genes in Abelson murine leukemia virus transformed pre-B cell lines.
    Nucleic Acids Res. 1989 Jul 11;17(13):5339-48 PMID: 2548157
  16. Initiation of transcription on nucleosomal templates.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9817-21 PMID: 2690074
  17. Transcriptionally active genome regions are preferred targets for retrovirus integration.
    J Virol. 1990 Feb;64(2):907-12 PMID: 2296087
  18. In vitro replication through nucleosomes without histone displacement.
    Nature. 1990 Feb 22;343(6260):719-26 PMID: 2304549
  19. Nucleosomes on linear duplex DNA allow homologous pairing but prevent strand exchange promoted by RecA protein.
    Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1344-8 PMID: 1996335
  20. Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.
    J Exp Med. 1991 Mar 1;173(3):711-20 PMID: 1900081
  21. Nucleosomes: regulators of transcription.
    Trends Genet. 1990 Dec;6(12):395-400 PMID: 2087781
  22. Superhelical stress and nucleosome-mediated repression of 5S RNA gene transcription in vitro.
    EMBO J. 1991 Nov;10(11):3419-28 PMID: 1717265
  23. Chromatin as an essential part of the transcriptional mechanism.
    Nature. 1992 Jan 16;355(6357):219-24 PMID: 1731219
  24. In vitro transcription through nucleosomes by T7 RNA polymerase.
    EMBO J. 1992 May;11(5):1941-7 PMID: 1582422
  25. V(D)J recombination on minichromosomes is not affected by transcription.
    J Biol Chem. 1992 Aug 5;267(22):15613-9 PMID: 1639801
  26. A transcriptionally active tRNA gene interferes with nucleosome positioning in vivo.
    Mol Cell Biol. 1992 Sep;12(9):4015-25 PMID: 1508199
  27. A nucleosome core is transferred out of the path of a transcribing polymerase.
    Cell. 1992 Oct 2;71(1):11-22 PMID: 1394427
  28. Isolation and physical characterization of a stable core particle.
    Nucleic Acids Res. 1980 Feb 25;8(4):767-79 PMID: 7433116
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.3.1949-1955.1994
Published
1994-03-00
Pages
1949-55
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358553
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.3.1949-1955.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2