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PMID: 8064222 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Coronavirus induction of class I major histocompatibility complex expression in murine astrocytes is virus strain specific.

The Journal of experimental medicine ·Vol. 180 ·No. 3 ·1994-09-01 ·Pages 1013-23

Gilmore W, Correale J, Weiner LP

Abstract

Neurotropic strains of mouse hepatitis viruses (MHV) such as MHV-A59 (A59) and MHV-4 (JHMV) cause acute and chronic encephalomyelitis and demyelination in susceptible strains of mice and rats. They are widely used as models of human demyelinating diseases such as multiple sclerosis (MS), in which immune mechanisms are thought to participate in the development of lesions in the central nervous system (CNS). The effects of MHV infection on target cell functions in the CNS are not well understood, but A59 has been shown to induce the expression of MHC class I molecules in glial cells after in vivo and in vitro infection. Changes in class I expression in infected cells may contribute to the immunopathogenesis of MHV infection in the CNS. In this communication, a large panel of MHV strains was tested for their ability to stimulate class I expression in primary astrocytes in vitro. The data show that the more hepatotropic strains, such as MHV-A59, MHV-1, MHV-2, MHV-3, MHV-D, MHV-K, and MHV-NuU, were potent inducers of class I expression in astrocytes during acute infection, measured by radioimmunoassay. The Kb molecule was preferentially expressed over Db. By contrast, JHMV and several viral strains derived from it did not stimulate the expression of class I molecules. Assays of virus infectivity indicated that the class I-inducing activity did not correlate with the ability of the individual viral strain to replicate in astrocytes. However, exposure of the viruses or the supernatants from infected astrocytes to ultraviolet light abolished the class I-inducing activity, indicating that infectious virus is required for class I expression. These data also suggest that class I expression was induced directly by virus infection, and not by the secretion of a soluble substance into the medium by infected astrocytes. Finally, analyses of A59/JHMV recombinant viral strains suggest that class I-inducing activity resides in one of the A59 structural genes.

MeSH Terms
Animals Astrocytes/immunology Cells, Cultured Coronavirus Infections/immunology Genes, Viral Histocompatibility Antigens Class I/biosynthesis Mice Mice, Inbred BALB C Mice, Inbred C57BL Murine hepatitis virus/genetics,immunology Species Specificity Virus Replication
Chemicals
Histocompatibility Antigens Class I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilmore W
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90033.
Correale J
Weiner L P
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.180.3.1013
Published
1994-09-01
Pages
1013-23
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191627
Subset
IM
Grants
NINDS NIH HHS · NS-18146-12 · United States
External Links
PubMed source DOI: 10.1084/jem.180.3.1013 PMC Full Text View journal details
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