Home LiteratureArticle Details
PMID: 1851765 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of brain-infiltrating mononuclear cells during infection with mouse hepatitis virus strain JHM.

Journal of neuroimmunology ·Vol. 32 ·No. 3 ·1991-06-00 ·Pages 199-207

Williamson JS, Sykes KC, Stohlman SA

Abstract

The eradication of infectious virus from the central nervous system (CNS) following infection with the neurotropic JHM strain of mouse hepatitis virus (JHMV) is thought to be immune-mediated. Furthermore, a significant decrease of infectious virus coincides with the appearance of prominent inflammatory infiltrates in the brain and spinal cord. In the present study, mononuclear cells infiltrating the brain during JHMV infection were isolated and characterized. While all subsets of immune cells were present, there appeared to be a temporal relationship between the peak incidence of CD8+ T cells (40% of total isolated cells) and reduction of virus at day 7 post-infection. Cells with the natural killer (NK) phenotype (at least 30%) were also present throughout infection. These data suggest that CD8+ T cells and NK cells are prominent among cells which infiltrate the brain during JHM virus infection and may have important roles in reduction of virus within the CNS.

MeSH Terms
Animals Brain/immunology,pathology G(M1) Ganglioside Glycosphingolipids/analysis Hepatitis, Viral, Animal/immunology,pathology Killer Cells, Natural/immunology Leukocytes, Mononuclear/immunology,pathology Lymphocytes/immunology,pathology Mice Mice, Inbred BALB C Murine hepatitis virus Phenotype T-Lymphocytes, Helper-Inducer/cytology T-Lymphocytes, Regulatory/cytology
Chemicals
Glycosphingolipids G(M1) Ganglioside asialo GM1 ganglioside
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williamson J S
Department of Neurology, University of Southern California School of Medicine, Los Angeles 90033.
Sykes K C
Stohlman S A
References (26)
26 references, click to expand
  1. Pathogenesis of demyelination induced by a mouse hepatitis.
    Arch Neurol. 1973 May;28(5):298-303 PMID: 4348723
  2. Natural killer cell activity during mouse hepatitis virus infection: response in the absence of interferon.
    Int J Cancer. 1983 Mar 15;31(3):309-14 PMID: 6186617
  3. Ganglio-N-tetraosylceramide (asialo GM1), an antigen common to the brain and immune system: its localization in myelin.
    Brain Res. 1985 May 13;334(1):117-24 PMID: 2581667
  4. Murine coronaviruses: isolation and characterization of two plaque morphology variants of the JHM neurotropic strain.
    J Gen Virol. 1982 Dec;63(2):265-75 PMID: 6296277
  5. Cytoimmunotherapy for persistent virus infection reveals a unique clearance pattern from the central nervous system.
    Nature. 1986 May 15-21;321(6067):239-43 PMID: 3086743
  6. Murine hepatitis virus-4 (strain JHM)-induced neurologic disease is modulated in vivo by monoclonal antibody.
    Virology. 1984 Jan 30;132(2):261-70 PMID: 6199889
  7. Susceptibility of astrocytes to class I MHC antigen-specific cytotoxicity.
    J Immunol. 1987 May 15;138(10):3254-8 PMID: 3106479
  8. Protective effect of monoclonal antibodies on lethal mouse hepatitis virus infection in mice.
    J Virol. 1986 Jul;59(1):168-71 PMID: 3012115
  9. Reactivity of anti-asialo GM1 serum with tumoricidal and non-tumoricidal mouse macrophages.
    J Leukoc Biol. 1985 May;37(5):597-614 PMID: 3884721
  10. Monoclonal antibodies to the matrix (E1) glycoprotein of mouse hepatitis virus protect mice from encephalitis.
    Virology. 1989 Jan;168(1):162-7 PMID: 2535900
  11. Immune mediated clearance of JHM virus from the central nervous system.
    Adv Exp Med Biol. 1987;218:399-410 PMID: 2829552
  12. In vivo effects of coronavirus-specific T cell clones: DTH inducer cells prevent a lethal infection but do not inhibit virus replication.
    J Immunol. 1986 Apr 15;136(8):3052-6 PMID: 2420882
  13. Studies on the mechanism of protection from acute viral encephalomyelitis by delayed-type hypersensitivity inducer T cell clones.
    J Neurol Sci. 1989 Apr;90(2):203-16 PMID: 2524553
  14. Effective clearance of mouse hepatitis virus from the central nervous system requires both CD4+ and CD8+ T cells.
    J Virol. 1990 Sep;64(9):4589-92 PMID: 2166833
  15. Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2).
    J Gen Virol. 1984 Nov;65 ( Pt 11):1931-42 PMID: 6209363
  16. Monocytes/macrophages isolated from the mouse central nervous system contain infectious Theiler's murine encephalomyelitis virus (TMEV).
    Virology. 1990 May;176(1):244-54 PMID: 2158691
  17. Identification of cytolytic lymphocytes in West Nile virus-infected murine central nervous system.
    J Gen Virol. 1989 Mar;70 ( Pt 3):565-73 PMID: 2543751
  18. Origin and fate of neural macrophages in a stab wound of the brain of the young rat.
    J Anat. 1987 Oct;154:215-27 PMID: 3446660
  19. Accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication.
    J Immunol. 1987 Feb 1;138(3):877-83 PMID: 3027167
  20. A glycolipid on the surface of mouse natural killer cells.
    Eur J Immunol. 1980 Mar;10(3):175-80 PMID: 6966574
  21. Identification of ganglio-N-tetraosylceramide as a new cell surface marker for murine natural killer (NK) cells.
    J Immunol. 1980 Jan;124(1):199-201 PMID: 6985637
  22. Establishment of monoclonal anti-Nk-1.1 antibody.
    Hybridoma. 1984 Fall;3(3):301-3 PMID: 6500587
  23. Establishment of cytotoxic T-cell clones specific for cells infected with mouse hepatitis virus.
    J Virol. 1988 Jul;62(7):2505-7 PMID: 2836629
  24. Human oligodendrocytes are susceptible to cytolysis by major histocompatibility complex class I-restricted lymphocytes.
    J Neuroimmunol. 1990 May;27(2-3):89-97 PMID: 1970580
  25. Flow cytometric analysis reveals the presence of asialo GM1 on the surface membrane of alloimmune cytotoxic T lymphocytes.
    J Immunol. 1986 Mar 1;136(5):1586-91 PMID: 2936802
  26. Mechanism of demyelination in JHM virus encephalomyelitis. Electron microscopic studies.
    Acta Neuropathol. 1973 Mar 30;24(1):76-85 PMID: 4349379
Article Info
Journal
Journal of neuroimmunology
Abbr.
J Neuroimmunol
ISSN
0165-5728
DOI
10.1016/0165-5728(91)90189-e
Published
1991-06-00
Pages
199-207
Language
English
Region
Netherlands
NLM ID
8109498
PMCID
PMC7119758
Subset
IM
Grants
NINDS NIH HHS · P01 NS018146 · United States
NINDS NIH HHS · NS18146 · United States
External Links
PubMed source DOI: 10.1016/0165-5728(91)90189-e PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2