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PMID: 8063852 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The COOH-terminal ends of internal signal and signal-anchor sequences are positioned differently in the ER translocase.

The Journal of cell biology ·Vol. 126 ·No. 5 ·1994-09-00 ·Pages 1127-32

Nilsson I, Whitley P, von Heijne G

Abstract

Signal peptides (SPs) target proteins to the secretory pathway and are cleaved from the nascent chain once the translocase in the ER has been engaged. Signal-anchor (SA) sequences also interact transiently with the ER translocase, but are not cleaved and move laterally out of the translocase to become permanent membrane anchors. One obvious difference between SP and SA sequences is the considerably longer hydrophobic regions (h regions) of the latter. To study the interaction between SP/SA sequences and the ER translocase, we have constructed signal sequences with poly-Leu h regions ranging in length from 8 to 29 residues and have characterized their locations within the translocase using both a new assay that measures the minimum number of amino acids needed to span the distance between the COOH-terminal end of the h region and the active site of the oligosaccharyl transferase enzyme and an assay where the efficiency of signal peptidase catalyzed cleavage is measured. Our results suggest that SP and SA sequences are positioned differently in the ER translocase.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,metabolism Base Sequence Biological Transport Endopeptidases/chemistry,metabolism Endoplasmic Reticulum/metabolism Escherichia coli Glycosylation Membrane Glycoproteins/chemistry,metabolism Membrane Proteins Molecular Sequence Data Protein Processing, Post-Translational Protein Sorting Signals/metabolism Ribosomes/metabolism Serine Endopeptidases Structure-Activity Relationship
Chemicals
Bacterial Proteins Membrane Glycoproteins Membrane Proteins Protein Sorting Signals Endopeptidases Serine Endopeptidases type I signal peptidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nilsson I
Karolinska Institute Center for Structural Biochemistry, NOVUM, Huddinge, Sweden.
Whitley P
von Heijne G
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36 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.126.5.1127
Published
1994-09-00
Pages
1127-32
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120157
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.126.5.1127 PMC Full Text View journal details
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