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PMID: 8058840 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

NPR genes, which are negatively regulated by phytochrome action in Lemna gibba L. G-3, can also be positively regulated by abscisic acid.

Plant physiology ·Vol. 105 ·No. 3 ·1994-07-00 ·Pages 949-54

Williams SA, Weatherwax SC, Bray EA, Tobin EM

Abstract

We have found that NPR1 and NPR2, two genes from Lemna gibba L. G-3 that can be negatively regulated by phytochrome action, can also be positively regulated by the plant hormone abscisic acid (ABA). Both genes were responsive to low concentrations of exogenous ABA; an increase in NPR1 RNA could be detected in response to concentrations as low as 10 nM. We have also tested phytochrome responsiveness of 5' promoter-deletion constructs of one of these genes, NPR1, in transient assays utilizing particle bombardment. This analysis demonstrated that DNA sequences important for phytochrome regulation are present downstream of -198 from the transcription start site. A response to ABA treatment could also be observed in the transient assay system. When intact plants were placed in darkness, there was an increase in ABA levels as well as increased levels of NPR1 and NPR2 RNA.

Related Genes
NPR NPR1 NPR2
MeSH Terms
Abscisic Acid/pharmacology Base Sequence Conserved Sequence Gene Expression Regulation/drug effects Genes, Plant Kinetics Molecular Sequence Data Phytochrome/metabolism Plants/drug effects,genetics,metabolism Promoter Regions, Genetic RNA, Messenger/analysis,biosynthesis Sequence Deletion
Chemicals
RNA, Messenger Phytochrome Abscisic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Williams S A
Department of Biology, University of California, Los Angeles 90024-1606.
Weatherwax S C
Bray E A
Tobin E M
References (17)
17 references, click to expand
  1. A negatively acting DNA sequence element mediates phytochrome-directed repression of phyA gene transcription.
    EMBO J. 1991 Oct;10(10):3015-24 PMID: 1915276
  2. An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089-93 PMID: 2902624
  3. Abscisic acid is involved in the iron-induced synthesis of maize ferritin.
    EMBO J. 1993 Feb;12(2):651-7 PMID: 8440255
  4. Abscisic Acid Negatively Regulates Expression of Chlorophyll a/b Binding Protein Genes during Soybean Embryogeny.
    Plant Physiol. 1991 Nov;97(3):1260-4 PMID: 16668519
  5. Regulation by ABA of beta-Conglycinin Expression in Cultured Developing Soybean Cotyledons.
    Plant Physiol. 1985 Nov;79(3):746-50 PMID: 16664485
  6. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  7. Gene expression in response to abscisic acid and osmotic stress.
    Plant Cell. 1990 Jun;2(6):503-12 PMID: 2152172
  8. Differential regulation of ABA-induced 23-25 kDa proteins in embryo and vegetative tissues of the viviparous mutants of maize.
    Plant Mol Biol. 1989 Oct;13(4):385-94 PMID: 2535256
  9. ABA-regulated gene expression: cis-acting sequences and trans-acting factors.
    Biochem Soc Trans. 1992 Feb;20(1):93-7 PMID: 1386045
  10. Sequence analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration stress in wheat.
    Plant Mol Biol. 1991 Jun;16(6):1073-6 PMID: 1830822
  11. Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding.
    Plant Cell. 1992 Apr;4(4):485-96 PMID: 1498606
  12. Hormonal and Environmental Regulation of the Carrot lea-Class Gene Dc3.
    Plant Physiol. 1992 Oct;100(2):576-81 PMID: 16653031
  13. Luciferase reporter gene cassettes for plant gene expression studies.
    Nucleic Acids Res. 1987 Oct 12;15(19):8115 PMID: 3671076
  14. Regulation of the maize rab17 gene promoter in transgenic heterologous systems.
    Plant Mol Biol. 1991 Nov;17(5):985-93 PMID: 1932688
  15. Isolation and Characterization of Three Genes Negatively Regulated by Phytochrome Action in Lemna gibba.
    Plant Physiol. 1991 Aug;96(4):1237-45 PMID: 16668325
  16. Molecular cloning and characterization of cold-regulated genes in barley.
    Plant Physiol. 1990 Aug;93(4):1504-10 PMID: 16667647
  17. Analysis of genes negatively regulated by phytochrome action in Lemna gibba and identification of a promoter region required for phytochrome responsiveness.
    Plant Physiol. 1993 Mar;101(3):915-24 PMID: 8310060
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.3.949
Published
1994-07-00
Pages
949-54
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC160745
Subset
IM
Grants
NIGMS NIH HHS · GM-23167 · United States
External Links
PubMed source DOI: 10.1104/pp.105.3.949 PMC Full Text View journal details
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