Sequence analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration stress in wheat.

Plant molecular biology ·Vol. 16 ·No. 6 ·1991-06-00 ·Pages 1073-6

Curry J, Morris CF, Walker-Simmons MK

Abstract

A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475-486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23,000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.

MeSH Terms

Abscisic Acid/pharmacology Amino Acid Sequence Base Sequence Blotting, Northern Cloning, Molecular Desiccation Gene Expression Regulation/drug effects,physiology Molecular Sequence Data Plant Proteins/chemistry,genetics Repetitive Sequences, Nucleic Acid/genetics Sequence Homology, Nucleic Acid Transcription, Genetic/physiology Triticum/drug effects,embryology,genetics

Chemicals

Plant Proteins late embryogenesis abundant protein, plant Abscisic Acid

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Curry J

U.S. Department of Agriculture, Washington State University, Pullman 99164-6420.

Morris C F

Walker-Simmons M K

References (9)

9 references, click to expand

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A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn.
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Molecular cloning and expression of abscisic Acid-responsive genes in embryos of dormant wheat seeds.
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Reduced Accumulation of ABA during Water Stress in a Molybdenum Cofactor Mutant of Barley.
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A comprehensive set of sequence analysis programs for the VAX.
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Article Info

Journal

Plant molecular biology

Abbr.

Plant Mol Biol

ISSN

0167-4412

DOI

10.1007/BF00016078

Published

1991-06-00

Pages

1073-6

Language

English

Region

Netherlands

NLM ID

9106343

Subset

Databases

GENBANK

X56882

External Links

PubMed source DOI: 10.1007/BF00016078 View journal details

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Sequence analysis of a cDNA encoding a group 3 LEA mRNA inducible by ABA or dehydration stress in wheat.

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