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PMID: 3575103 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The nucleotide sequence of a cDNA clone encoding the wheat Em protein.

Nucleic acids research ·Vol. 15 ·No. 8 ·1987-04-24 ·Pages 3607-18

Litts JC, Colwell GW, Chakerian RL, Quatrano RS

Abstract

The nucleotide sequence of a cDNA clone isolated from developing wheat embryos and encoding the Em protein is reported. The entire coding region for Em and the 3' non-translated flank are contained within this clone. The amino acid sequence deduced for Em is very rich in glycine (18 mol%) as well as both basic and acidic residues. The molecular weight of the protein is ca. 9,900 daltons. The deduced sequence is supported by direct amino acid sequencing of cyanogen bromide cleavage fragments obtained from purified Em protein. Em is shown by Southern blots to be a product of a gene family of approximately ten members.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics DNA, Recombinant Fabaceae/genetics Genes Plant Growth Regulators Plant Proteins/genetics Plants, Medicinal Species Specificity Triticum/embryology,genetics
Chemicals
DNA, Recombinant E(M) protein, Triticum aestivum Plant Growth Regulators Plant Proteins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Litts J C
Colwell G W
Chakerian R L
Quatrano R S
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.8.3607
Published
1987-04-24
Pages
3607-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340753
Subset
IM
Databases
GENBANK
Y00123
External Links
PubMed source DOI: 10.1093/nar/15.8.3607 PMC Full Text View journal details
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