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PMID: 8051020 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Identification and structure of the nasR gene encoding a nitrate- and nitrite-responsive positive regulator of nasFEDCBA (nitrate assimilation) operon expression in Klebsiella pneumoniae M5al.

Journal of bacteriology ·Vol. 176 ·No. 16 ·1994-08-00 ·Pages 5077-85

Goldman BS, Lin JT, Stewart V

Abstract

Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by general nitrogen control and also by nitrate or nitrite induction. We have identified a gene, nasR, that is necessary for nitrate and nitrite induction. The nasR gene, located immediately upstream of the nasFEDCBA operon, encodes a 44-kDa protein. The NasR protein shares carboxyl-terminal sequence similarity with the AmiR protein of Pseudomonas aeruginosa, the positive regulator of amiE (aliphatic amidase) gene expression. In addition, we present evidence that the nasF operon is not autogenously regulated.

Related Genes
amiR nadB nasA nasC nasD nasE nasF nasR
MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence DNA-Binding Proteins/metabolism Gene Expression Regulation, Bacterial Genes, Bacterial Genes, Regulator Klebsiella pneumoniae/genetics Molecular Sequence Data Mutagenesis, Insertional Nitrates/metabolism Nitrites/metabolism Operon Trans-Activators/genetics Transcriptional Activation
Chemicals
Bacterial Proteins DNA-Binding Proteins Nitrates Nitrites Trans-Activators nasR protein, Klebsiella pneumoniae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Goldman B S
Sections of Microbiology, Cornell University, Ithaca, New York 14853-8101.
Lin J T
Stewart V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.16.5077-5085.1994
Published
1994-08-00
Pages
5077-85
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196347
Subset
IM
Databases
GENBANK
L27824
External Links
PubMed source DOI: 10.1128/jb.176.16.5077-5085.1994 PMC Full Text View journal details
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