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PMID: 8361359 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification of an operon involved in the assimilatory nitrate-reducing system of Azotobacter vinelandii.

Molecular microbiology ·Vol. 8 ·No. 6 ·1993-06-00 ·Pages 1145-53

Ramos F, Blanco G, Gutiérrez JC, Luque F, Tortolero M

Abstract

A number of mutants lacking nitrate reductase (Nas-) or nitrite reductase (Nis-) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned from an Azotobacter vinelandii gene bank. nasA encodes for nitrite reductase apoenzyme, whereas nasB is specific for nitrate reductase activity. Nitrate reductase exerts a regulatory effect on nasAB.

Related Genes
nas nasA nasB nis ntm ntrB ntrC
MeSH Terms
Amino Acid Sequence Apoenzymes/genetics,metabolism Azotobacter vinelandii/enzymology,genetics Bacterial Proteins/genetics,metabolism Enzyme Induction Escherichia coli/enzymology,genetics Gene Expression Regulation, Bacterial Molecular Sequence Data Nitrate Reductase Nitrate Reductases/genetics,metabolism Nitrates/metabolism Nitrite Reductases/genetics,metabolism Operon Oxidation-Reduction Recombinant Fusion Proteins/metabolism Sequence Alignment Sequence Homology, Amino Acid
Chemicals
Apoenzymes Bacterial Proteins Nitrates Recombinant Fusion Proteins Nitrate Reductases Nitrite Reductases Nitrate Reductase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ramos F
Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Spain.
Blanco G
Gutiérrez J C
Luque F
Tortolero M
Article Info
Journal
Molecular microbiology
Abbr.
Mol Microbiol
ISSN
0950-382X
DOI
10.1111/j.1365-2958.1993.tb01659.x
Published
1993-06-00
Pages
1145-53
Language
English
Region
England
NLM ID
8712028
Subset
IM
External Links
PubMed source DOI: 10.1111/j.1365-2958.1993.tb01659.x View journal details
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