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PMID: 1400180 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molybdenum cofactor (chlorate-resistant) mutants of Klebsiella pneumoniae M5al can use hypoxanthine as the sole nitrogen source.

Journal of bacteriology ·Vol. 174 ·No. 19 ·1992-10-00 ·Pages 6298-302

Garzón A, Li J, Flores A, Casadesus J, Stewart V

Abstract

Selection for chlorate resistance yields mol (formerly chl) mutants with defects in molybdenum cofactor synthesis. Complementation and genetic mapping analyses indicated that the Klebsiella pneumoniae mol genes are functionally homologous to those of Escherichia coli and occupy analogous genetic map positions. Hypoxanthine utilization in other organisms requires molybdenum cofactor as a component of xanthine dehydrogenase, and thus most chlorate-resistant mutants cannot use hypoxanthine as a sole source of nitrogen. Surprisingly, the K. pneumoniae mol mutants and the mol+ parent grew equally well with hypoxanthine as the sole nitrogen source, suggesting that K. pneumoniae has a molybdenum cofactor-independent pathway for hypoxanthine utilization.

Related Genes
moa mod moe mog
MeSH Terms
Chromosome Mapping Coenzymes/genetics Gene Expression Regulation, Bacterial Genetic Complementation Test Hypoxanthine Hypoxanthines/metabolism Klebsiella pneumoniae/genetics,metabolism Metalloproteins/metabolism Molybdenum/metabolism Molybdenum Cofactors Mutagenesis Nitrate Reductase Nitrate Reductases/analysis Nitrogen/metabolism Pteridines/metabolism Transduction, Genetic Tungsten/pharmacology Tungsten Compounds
Chemicals
Coenzymes Hypoxanthines Metalloproteins Molybdenum Cofactors Pteridines Tungsten Compounds Hypoxanthine Molybdenum molybdenum cofactor Nitrate Reductases Nitrate Reductase Nitrogen tungstate Tungsten
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Garzón A
Departamento de Genética, Universidad de Sevilla, Spain.
Li J
Flores A
Casadesus J
Stewart V
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.19.6298-6302.1992
Published
1992-10-00
Pages
6298-302
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207701
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.19.6298-6302.1992 PMC Full Text View journal details
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