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PMID: 2832370 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification and expression of genes narL and narX of the nar (nitrate reductase) locus in Escherichia coli K-12.

Journal of bacteriology ·Vol. 170 ·No. 4 ·1988-04-00 ·Pages 1589-97

Stewart V, Parales J

Abstract

Previous studies have shown that narL+ is required for nitrate induction of nitrate reductase synthesis and for nitrate inhibition of fumarate reductase synthesis in Escherichia coli. We cloned narL on a 5.1-kilobase HindIII fragment. Our clone also contained a previously unidentified gene, which we propose to designate as narX, as well as a portion of narK. Maxicell experiments indicated that narL and narX encode proteins with approximate MrS of 28,000 and 66,000, respectively. narX insertion mutations reduced nitrate reductase structural gene expression by less than twofold. Expression of phi (narL-lacZ) operon fusions was weakly induced by nitrate but was indifferent to aerobiosis and independent of fnr. Expression of phi (narX-lacZ) operon fusions was induced by nitrate and was decreased by narL and fnr mutations. A phi (narK-lacZ) operon fusion was induced by nitrate, and its expression was fully dependent on narL+ and fnr+. Analysis of these operon fusions indicated that narL and narX are transcribed counterclockwise with respect to the E. coli genetic map and that narK is transcribed clockwise.

MeSH Terms
Cloning, Molecular Culture Media DNA Restriction Enzymes Escherichia coli/enzymology,genetics Gene Expression Regulation Genes, Bacterial Genes, Regulator Genetic Complementation Test Mutation Nitrate Reductases/biosynthesis,genetics Nitrates/metabolism Operon Plasmids Transcription, Genetic
Chemicals
Culture Media Nitrates Nitrate Reductases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stewart V
Department of Microbiology, Cornell University, Ithaca, New York 14853.
Parales J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.170.4.1589-1597.1988
Published
1988-04-00
Pages
1589-97
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC211006
Subset
IM
Grants
NIGMS NIH HHS · GM36877 · United States
External Links
PubMed source DOI: 10.1128/jb.170.4.1589-1597.1988 PMC Full Text View journal details
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