Abstract
We have identified a gene that acts in trans to activate the expression of the phenazine biosynthetic genes in the biological control organism Pseudomonas aureofaciens 30-84. This gene, phzR (phenazine regulator), is located upstream of and divergently transcribed from the phenazine biosynthetic genes. Thus, the phenazine biosynthetic locus consists of at least two divergently transcribed operons. A functional phzR gene is required for phenazine production. The nucleotide sequence of phzR revealed an open reading frame of 723 nucleotides encoding a protein of ca. 27 kDa. The predicted amino acid sequence of PhzR has homology with other bacterial positive transcriptional activators, including LasR of Pseudomonas aeruginosa, LuxR of Vibrio fischerii, and TraR of Agrobacterium tumefaciens. The addition of cell-free supernatants from late-exponential-phase cultures of strain 30-84 resulted in expression of a genomic phzB:lacZ reporter strain at a lower cell density than normal, indicating the possible presence of an autoinducer. These results indicate that PhzR is a member of a two-component sensor-regulator family with known or predicted carboxy-terminal DNA-binding domains which regulates gene expression in response to environmental and cell density signals.
MeSH Terms
Amino Acid Sequence
Anti-Bacterial Agents/biosynthesis
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA Mutational Analysis
DNA-Binding Proteins/genetics
Escherichia coli/genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial/genetics
Genes, Regulator/genetics
Molecular Sequence Data
Phenazines/metabolism
Pseudomonas/growth & development,metabolism
Recombinant Proteins/biosynthesis
Sequence Analysis, DNA
Sequence Deletion
Sequence Homology, Amino Acid
Trans-Activators/genetics
Transcriptional Activation
Chemicals
Anti-Bacterial Agents
Bacterial Proteins
DNA-Binding Proteins
Phenazines
PhzR protein, Pseudomonas
Recombinant Proteins
Trans-Activators
1-phenazinecarboxylic acid
2-hydroxyphenazine-1-carboxylic acid
2-hydroxyphenazine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pierson L S
Department of Plant Pathology, University of Arizona, Tucson 85721.
Keppenne V D
Wood D W
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