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PMID: 7945214 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structure of the glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase.

The Biochemical journal ·Vol. 302 ( Pt 3) ·1994-09-15 ·Pages 861-5

Redman CA, Thomas-Oates JE, Ogata S, Ikehara Y, Ferguson MA

Abstract

The glycosylphosphatidylinositol membrane anchor of human placental alkaline phosphatase was isolated by exhaustive proteolysis followed by hydrophobic interaction chromatography. The resulting glycosylphosphatidylinositol-peptide was subjected to compositional analysis and chemical and enzymic modifications. The neutral-glycan fraction, prepared by dephosphorylation followed by HNO2 deamination and reduction, was sequenced using exoglycosidases and acetolysis. The phosphatidylinositol moiety was analysed by fast-atom bombardment mass spectrometry and gas chromatography-mass spectrometry. Taken together the data suggest the structure, Thr-Asp-ethanolamine-PO4-Man alpha 1-2Man alpha 1-6Man alpha 1-4GlcN-(sn-1-O- alkyl-2-O-acylglycerol-3-PO4-1-myo-D-inositol), which contains an additional ethanolamine phosphate group at an unknown position.

MeSH Terms
Alkaline Phosphatase/chemistry Carbohydrate Sequence Female Gas Chromatography-Mass Spectrometry Glycosylphosphatidylinositols/chemistry Humans Molecular Sequence Data Placenta/enzymology Spectrometry, Mass, Fast Atom Bombardment
Chemicals
Glycosylphosphatidylinositols Alkaline Phosphatase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Redman C A
Department of Biochemistry, University of Dundee, U.K.
Thomas-Oates J E
Ogata S
Ikehara Y
Ferguson M A
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj3020861
Published
1994-09-15
Pages
861-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1137310
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj3020861 PMC Full Text View journal details
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