Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 5 ·1988-03-00 ·Pages 1398-402

Micanovic R, Bailey CA, Brink L, Gerber L, Pan YC, Hulmes JD, Udenfriend S

Abstract

A carboxyl-terminal chymotryptic peptide from mature human placental alkaline phosphatase was purified by HPLC and monitored by a specific RIA. Sequencing and amino acid assay showed that the carboxyl terminus of the peptide was aspartic acid, representing residue 484 of the proenzyme as deduced from the corresponding cDNA. Further analysis of the peptide showed it to be a peptidoglycan containing one residue of ethanolamine, one residue of glucosamine, and two residues of neutral hexose. The inositol glycan is apparently linked to the alpha carboxyl group of the aspartic acid through the ethanolamine. Location of the inositol glycan on Asp-484 of the proenzyme indicates that a 29-residue peptide is cleaved from the nascent protein during the post-translational condensation with the phosphatidylinositol-glycan.

MeSH Terms

Alkaline Phosphatase/metabolism Aspartic Acid/metabolism Humans In Vitro Techniques Membrane Proteins/metabolism Peptide Fragments/analysis Peptidoglycan/metabolism Phosphatidylinositols/metabolism Placenta/enzymology Protein Processing, Post-Translational

Chemicals

Membrane Proteins Peptide Fragments Peptidoglycan Phosphatidylinositols Aspartic Acid Alkaline Phosphatase

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Micanovic R

Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110.

Bailey C A

Brink L

Gerber L

Pan Y C

Hulmes J D

Udenfriend S

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.5.1398

Published

1988-03-00

Pages

1398-402

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC279778

Subset

External Links

PubMed source DOI: 10.1073/pnas.85.5.1398 PMC Full Text View journal details

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Aspartic acid-484 of nascent placental alkaline phosphatase condenses with a phosphatidylinositol glycan to become the carboxyl terminus of the mature enzyme.

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