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PMID: 7915036 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Structure and expression of an Arabidopsis acetyl-coenzyme A carboxylase gene.

Plant physiology ·Vol. 105 ·No. 2 ·1994-06-00 ·Pages 611-7

Roesler KR, Shorrosh BS, Ohlrogge JB

Abstract

Acetyl-coenzyme A carboxylase (ACCase) catalyzes the formation of malonyl-coenzyme A, which is used in the plastid for fatty acid synthesis and in the cytosol for several pathways including fatty acid elongation and flavonoid synthesis. Two overlapping Arabidopsis genomic clones were isolated and sequenced to determine the entire ACCase-coding region. Thirty introns with an average size of 94 bp were identified by comparison with an alfalfa ACCase cDNA sequence. The 10-kb Arabidopsis ACCase gene encodes a 251-kD polypeptide, which has 80% amino acid sequence identity with alfalfa ACCase and about 40% identity with ACCase of rat, chicken, yeast, and the diatom Cyclotella. No chloroplast transit peptide sequence was observed, suggesting that this Arabidopsis gene encodes a cytosolic ACCase isozyme. ACCase gene transcripts were detected by RNase protection assays in Arabidopsis root, leaf, silique, and seed. Genomic DNA blot analysis revealed the presence of a second related Arabidopsis ACCase gene.

Related Genes
ACCase
MeSH Terms
Acetyl-CoA Carboxylase/genetics Amino Acid Sequence Animals Arabidopsis/enzymology,genetics Chromosome Mapping Cytosol/enzymology DNA, Complementary/genetics Gene Expression Genes, Plant Isoenzymes/genetics Medicago sativa/enzymology,genetics Molecular Sequence Data Multigene Family Rats Sequence Homology, Amino Acid Species Specificity
Chemicals
DNA, Complementary Isoenzymes Acetyl-CoA Carboxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Roesler K R
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824.
Shorrosh B S
Ohlrogge J B
References (20)
20 references, click to expand
  1. Analysis of Glucocerebrosides of Rye (Secale cereale L. cv Puma) Leaf and Plasma Membrane.
    Plant Physiol. 1991 Jan;95(1):58-68 PMID: 16667981
  2. Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco.
    Proc Natl Acad Sci U S A. 1992 Dec 1;89(23):11184-8 PMID: 1454797
  3. Chloroplast-encoded protein as a subunit of acetyl-CoA carboxylase in pea plant.
    J Biol Chem. 1993 Nov 25;268(33):25118-23 PMID: 7901221
  4. Role of reversible phosphorylation of acetyl-CoA carboxylase in long-chain fatty acid synthesis.
    FASEB J. 1989 Sep;3(11):2250-6 PMID: 2570725
  5. Wheat acetyl-CoA carboxylase.
    Plant Mol Biol. 1993 Jun;22(3):547-52 PMID: 8101104
  6. Molecular cloning, characterization, and elicitation of acetyl-CoA carboxylase from alfalfa.
    Proc Natl Acad Sci U S A. 1994 May 10;91(10):4323-7 PMID: 7910406
  7. Fat metabolism in higher plants. LIV. A procaryotic type acetyl CoA carboxylase in spinach chloroplasts.
    Arch Biochem Biophys. 1972 Sep;152(1):83-91 PMID: 4561257
  8. Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants.
    EMBO J. 1991 Sep;10(9):2635-44 PMID: 1868837
  9. Cloning of the yeast FAS3 gene and primary structure of yeast acetyl-CoA carboxylase.
    Proc Natl Acad Sci U S A. 1992 May 15;89(10):4534-8 PMID: 1350093
  10. Characterization of Maize Acetyl-Coenzyme A Carboxylase.
    Plant Physiol. 1993 Feb;101(2):499-506 PMID: 12231704
  11. Improved purification and further characterization of acetyl-CoA carboxylase from cultured cells of parsley (Petroselinum hortense).
    Eur J Biochem. 1983 Jun 15;133(2):335-9 PMID: 6133748
  12. Evolutionary conservation among biotin enzymes.
    J Biol Chem. 1988 May 15;263(14):6461-4 PMID: 2896195
  13. Cloning and characterization of the gene that encodes acetyl-coenzyme A carboxylase in the alga Cyclotella cryptica.
    J Biol Chem. 1993 Sep 15;268(26):19254-9 PMID: 8103514
  14. Phytoalexin synthesis in soybean cells: elicitor induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs and correlation with phytoalexin accumulation.
    Arch Biochem Biophys. 1984 Jul;232(1):240-8 PMID: 6540068
  15. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  16. Expression of the Acc1 Gene-Encoded Acetyl-Coenzyme A Carboxylase in Developing Maize (Zea mays L.) Kernels.
    Plant Physiol. 1993 Mar;101(3):1097-1101 PMID: 12231761
  17. An inspection of the domain between putative TATA box and translation start site in 79 plant genes.
    Nucleic Acids Res. 1987 Aug 25;15(16):6643-53 PMID: 3628002
  18. Primary structure of chicken liver acetyl-CoA carboxylase deduced from cDNA sequence.
    J Biol Chem. 1988 Feb 25;263(6):2651-7 PMID: 2893793
  19. Acetyl-coenzyme A carboxylase from the developing endosperm of Ricinus communis. I. Isolation and characterization.
    Arch Biochem Biophys. 1983 Sep;225(2):576-85 PMID: 6137995
  20. Detection of single base substitutions by ribonuclease cleavage at mismatches in RNA:DNA duplexes.
    Science. 1985 Dec 13;230(4731):1242-6 PMID: 4071043
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.105.2.611
Published
1994-06-00
Pages
611-7
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC159401
Subset
IM
Databases
GENBANK
L27074
External Links
PubMed source DOI: 10.1104/pp.105.2.611 PMC Full Text View journal details
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