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PMID: 8101104 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Wheat acetyl-CoA carboxylase.

Plant molecular biology ·Vol. 22 ·No. 3 ·1993-06-00 ·Pages 547-52

Gornicki P, Haselkorn R

Abstract

The acetyl-CoA carboxylase present in both wheat germ and total wheat leaf protein contains ca. 220 kDa subunits. It is the major biotin-dependent carboxylase present in wheat chloroplasts. Active acetyl-CoA carboxylase purified from wheat germ is a homodimer with an apparent molecular mass of ca. 500 kDa. The enzyme from wheat germ or from wheat chloroplasts is sensitive to the herbicide haloxyfop at micromolar levels. The incorporation of 14C-acetate into fatty acids in freshly cut wheat seedling leaves provides a convenient in vivo assay for both acetyl-CoA carboxylase and haloxyfop.

MeSH Terms
Acetyl-CoA Carboxylase/chemistry,drug effects,isolation & purification Amino Acid Sequence Herbicides/pharmacology Molecular Sequence Data Pyridines/pharmacology Triticum/enzymology
Chemicals
Herbicides Pyridines haloxyfop Acetyl-CoA Carboxylase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gornicki P
Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.
Haselkorn R
References (14)
14 references, click to expand
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
Published
1993-06-00
Pages
547-52
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
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