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PMID: 7910406 Published · ppublish English Comparative Study Journal Article

Molecular cloning, characterization, and elicitation of acetyl-CoA carboxylase from alfalfa.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 10 ·1994-05-10 ·Pages 4323-7

Shorrosh BS, Dixon RA, Ohlrogge JB

Abstract

Acetyl-CoA carboxylase [ACCase; acetyl-CoA:carbon-dioxide ligase (ADP-forming), EC 6.4.1.2] catalyzes the ATP-dependent carboxylation of acetyl CoA to produce malonyl CoA. In plants, malonyl CoA is needed for plastid localized fatty acid biosynthesis and for a variety of pathways in the cytoplasm including flavonoid biosynthesis. We have determined the full nucleotide sequence of an ACCase from alfalfa, which appears to represent a cytoplasmic isozyme. Partial cDNAs were isolated from a cDNA library of suspension culture cells that had been elicited for isoflavonoid phytoalexin synthesis. The full-length sequence was obtained by primer extension and amplification of the cDNA with synthetic primers. The sequence codes for a protein of 2257 amino acids with a calculated M(r) of 252,039. The biotin carboxylase, biotin carboxyl carrier protein, and carboxyltransferase domains, respectively, show approximately 72%, 50%, and 65% sequence similarity to those of animal, diatom, and yeast ACCase sequences. ACCase enzyme activity and transcripts are induced severalfold upon addition of yeast or fungal elicitors to alfalfa cell cultures.

MeSH Terms
Acetyl-CoA Carboxylase/biosynthesis,isolation & purification,metabolism Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Primers DNA, Complementary/analysis Gene Expression Genomic Library Medicago sativa/enzymology,genetics Molecular Sequence Data Molecular Weight Oligodeoxyribonucleotides Rats Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA Primers DNA, Complementary Oligodeoxyribonucleotides Acetyl-CoA Carboxylase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shorrosh B S
Department of Botany and Plant Pathology, Michigan State University, East Lansing 48824-1312.
Dixon R A
Ohlrogge J B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.10.4323
Published
1994-05-10
Pages
4323-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43777
Subset
IM
Databases
GENBANK
L25042
External Links
PubMed source DOI: 10.1073/pnas.91.10.4323 PMC Full Text View journal details
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